40分钟深低温停循环大鼠长期存活模型的建立

A long-term survival model of 40-min deep hypothermic circulatory arrest in rats

目的建立高生存率的大鼠深低温停循环(DHCA)模型,为研究DHCA相关器官损伤及保护机制奠定基础。方法选取20只SD大鼠,随机(随机数字法)分为实验组(n=10)和对照组(n=10),麻醉后,两组大鼠均采用大鼠尾动脉插管灌注,右颈静脉插管引流,左股浅动脉插管监测血压的插管方式,实验组开始体外循环后降温至脑温18℃,开始DHCA 40 min,随后复温至34℃停机。对照组只插管不行体外循环。各组均分别于转机前,术中15 min,复温后10 min,复温40 min,停机后30 min 5个时间点抽取血气分析,记录各时间点血气值,并于术中实时监测记录血流动力学参数。结果DHCA组大鼠有一只死亡,其余大鼠均术后存活。DHCA组较对照组术中乳酸上升明显(DHCA组:7.84 mmol/L,对照组:1.93 mmol/L,P<0.05)。结论本研究成功建立了40 min DHCA大鼠长期存活模型。

Objective To establish a long-term survival model of deep hypothermic circulatory arrest(DHCA)in rats,which could contribute to the research of organ damage mechanism and clinical treatments related to DHCA.Methods Twenty Sprague-Dawley rats were randomly(random number)divided into the sham group(n=10)and DHCA group(n=10).After anesthesia,a 20 G catheter was cannulated in the tail artery for arterial inflow,a multiorificed catheter in the right jugular vein for venous drainage,and a 24G catheter in the branch of left femoral artery for artery blood pressure monitoring.Rats in the DHCA group underwent DHCA procedure for 40 min after brain temperature cooled to 18℃,then rewarmed for 40 min,till the brain temperature were above 34℃.Rats in the sham group were cannulated but did not undergo cardiopulmonary bypass(CPB).Hemodynamic parameters and blood gas analysis were measured for 5 times(pre-CPB,15 min after CPB,10 min after rewarming,40 min after rewarming,and 30 min after CPB).Results One rat in the DHCA group died,and the rest rats survived.The lactate level in the DHCA group after rewarming during operation was significantly higher than that in the sham group(7.84 mmol/L vs 1.93 mmol/L,P<0.05).Conclusions In this study,40-min DHCA model in rats is characterized by safe and long-term survival.