内源性血红素氧合酶-1/一氧化碳系统及消退素E1在克罗恩病患者中的表达

Expression of endogenous heme oxygenase-1/carbon monoxide system and resolvin E1 in the patients of Crohn disease

目的探讨克罗恩病(CD)患者血红素氧合酶-1(HO-1)/一氧化碳(CO)系统及消退素E1(RvE1)的表达,探讨其在CD发病机制中的可能作用。方法采用SABC法观察HO-1在40例CD患者(根据CD活动指数评分分为活动期CD 20例及缓解期CD 20例)及15例健康对照组结肠黏膜组织中的定位,结肠黏膜中HO-1平均吸光度值通过病理图文分析系统计算。采用比色法检测结肠黏膜中CO含量。通过酶联免疫吸附法(ELISA)测出血清中RvE1的含量。结果活动期CD组结肠黏膜上皮细胞中HO-1表达明显,且活动期CD患者结肠黏膜中的HO-1吸光度值明显高于缓解期CD组及健康对照组(0.358±0.021比0.163±0.018和0.177±0.032)(P<0.01)。结肠黏膜中CO浓度在活动期CD组中的表达明显高于缓解期CD组和健康对照组[(23.79±1.81)μmol/g比(18.68±1.64)和(17.13±1.23)μmol/g](P<0.01)。活动期CD组血清中RvE1含量明显高于缓解期CD组和健康对照组[(41.35±2.76)ng/ml比(15.34±2.19)和(14.51±1.47)ng/ml](P<0.01)。结论内源性HO-1/CO系统、RvE1等新型抗炎介质在活动期CD患者中的异常表达表明其在CD的发病机制中可能起到重要的作用。

Objective To investigated the change of endogenous heme oxygenase-1(HO-1)/carbon monoxide(CO)system and resolvin E1(RvE1)in patients with crohn disease(CD),and its effects on the pathogenesis of ulcerative colitis.MethodsThe distribution and expression of HO-1 proteins in the colonic mucous in 40 cases with CD and 15 cases of normal control group were detected by SABC immunohistochemistry.The average optical density value of HO-1 was analyzed with an Image Analyzing systems.The concentration of CO in the colonic mucosa was detected with chemical methods.The levels of RvE1 in serum was detected by enzyme linked immunosorbent assay(ELISA).ResultsThe expression of HO-1 proteins in patients with active CD were significantly higherin the colonic mucous membrane epithelia.The average optical density value of HO-1 in patients with active CD was higher than that in normal group and remission of CD(0.358±0.021 vs.0.163±0.018 and 0.177±0.032)(P<0.01).The concentrations of CO in active CD was significantly higher than that in the normal control group and remission of CD[(23.79±1.81)μmol/g vs.(18.68±1.64)and(17.13±1.23)μmol/g](P<0.01).The levels of RvE1 in patients with active CD were significantly higher than those in normal group and remission of CD[(41.35±2.76)ng/ml vs.(15.34±2.19)and(14.51±1.47)ng/ml](P<0.01).ConclusionsThe abnormal expression of HO-1/CO system and RvE in activity of the CD may play an important role in the pathogenesis of CD.