摘要
构建表达H9N2亚型禽流感病毒HA2蛋白的延迟裂解型重组减毒沙门菌χ11218(pYA4545-HA2-DEC205-sec)和χ11218(pYA4545-HA2-DEC205-dimer-sec),将其免疫小鼠,通过流式细胞术检测免疫细胞的变化。结果表明,沙门菌χ11218(pYA4545-HA2-DEC205-dimer-sec)能够提高CD3+CD4+T细胞分泌细胞因子IL-4、IFN-γ的水平。这说明重组沙门菌免疫小鼠能够激活机体的免疫系统,诱导发生一定的免疫反应。
The regulated delayed lysis recombinant attenuated Salmonella χ11218(pYA4545-HA2-DEC-205-sec)and χ1218(pYA4545-HA2-DEC205-dimer-sec)expressing HA2 protein of H9N2 subtype avian influenza virus were constructed.Mice were immunized with both strains and the immune responses were determined by flow cytometry.The results showed that the recombinant Salmonella strains could increase the levels of IL-4 and IFN-y secreted by CD3^+CD4^+T cells,indicating that the immunization with recombinant Salmonella could activate host immune system and induce the occurrence of certain immune responses.
作者
胡译文
刘晶
赵维静
谢静
许可
杨桂连
姜延龙
王春凤
HU Yi-wen;LIU Jing;ZHAO Wei-jing;XIE Jing;XU Ke;YANG Gui-lian;JIANG Yan-long;WANG Chun-feng(College of Animal Science and Technology,Jilin Provincial Engineering Research Center of Animal Probiotics、Key Laboratory of Animal Production and Quality Security,Ministry of Education、Jilin Agricultural University,Changchun 130118 China)
出处
《中国兽医科学》
CAS
CSCD
北大核心
2019年第4期453-460,共8页
Chinese Veterinary Science
基金
国家"十三五"重点研发计划项目(2017YFD0501000
2017YFD0500400)
国家自然科学基金项目(31602092
31672528)
吉林省教育厅科学技术研究项目(JJKH20170316KJ)
吉林省科技发展计划项目(20160519011JH
20170204034NY
20180201040NY)
吉林省产业创新专项资金项目(2016C063)
关键词
禽流感病毒
HA2
延迟裂解
重组减毒沙门菌
avian influenza virus
HA2
delayed lytic
recombinant attenuated Salmonella
