摘要
以冷藏凡纳滨对虾优势腐败菌(希瓦氏菌、沙雷氏菌、气单胞菌)为研究对象,分析研究腐胺产量以及外源腐胺对其生长及代谢的影响。结果表明,气单胞菌(Aer)不具有产生腐胺的能力,而希瓦氏菌(She)和沙雷氏菌(Ser)都具有产生腐胺的能力,且希瓦氏菌积累腐胺的能力远高于沙雷氏菌。外源添加低浓度的腐胺(1 mmol/L)显著促进了希瓦氏菌的生长和生物膜的形成,但是高浓度的腐胺失去其促进生长的作用。腐胺对沙雷氏菌(Ser)和气单胞菌(Aer)生长和生物膜均无影响。L--二氟甲基鸟氨酸盐(DFMO,10 mmol/L)可抑制希瓦氏菌腐胺的产生,同时显著抑制希瓦氏菌的生长,而通过回补一定量的腐胺(1 mmol/L)可恢复其生长。结果表明,腐胺与希瓦氏菌成为优势腐败菌有紧密联系,此研究可为深入探究希瓦氏菌的致腐机制提供理论依据。
Effects of putrescine on the growth and biofilm of the spoiled strains(Shewanella spp., Serratia spp., Aeromonas spp.) isolated from refrigerated shrimp were investigated. The results showed that Aeromonas spp.(Aer) did not have the ability to produce putrescine, while Shewanella spp.(She) and Serratia spp.(Ser)had the ability to produce putrescine. Low concentration of putrescine(1 mmol/L)significantly promoted the growth and biofilm formation of She, but high concentrations of putrescine lost its growth-promoting effect. However, putrescine had no effect on the growth and biofilm of Ser and Aer.L-ɑ-difluoromethylornithinate(DFMO, 10 mmol/L), which reduced the ability of She to produce putrescine to a certain extent, significantly inhibited the growth of She, and exogenous addition of putrescine(1 mmol/L) restored its growth. The results indicated that putrescine might play an important role in the Shewanella spp being the dominant spoiled bacteria, which will provide a basis for study of the spoiled mechanism of Shewanella.
作者
于红雷
史国萃
常晶
曾名湧
刘尊英
YU Honglei;SHI Guocui;CHANG Jing;ZENG Mingyong;LIU Zunying(College of Food Science and Engineering, Ocean University of China, Qingdao 266003)
出处
《食品科技》
CAS
北大核心
2019年第3期131-135,共5页
Food Science and Technology
基金
国家自然科学基金项目(31671919)
宁波市农业重大项目(2017C110006)