摘要
Cell adhesion is the basis for some cell isolation methods, and is influenced by both of the biochemical and topographic characteristics of the substrates. Herein, based on cell imprinting and click chemistry,we have developed a cell-imprinted polydimethylsiloxane(PDMS) with aptamer functionalization(APTCIS). The atom force microscopic analysis results showed that the hierarchical structure matching well with the target cells is successfully introduced on the surface of the APT-CIS. By using the synergistic effects of hierarchical structure and aptamer affinity, the APT-CIS was successfully used for the selective cell adhesion, and 93.9% ± 0.8% of the captured cells could then be released. Thus, the APT-CIS holds promise in selective cell isolation and sorting fields.
Cell adhesion is the basis for some cell isolation methods, and is influenced by both of the biochemical and topographic characteristics of the substrates. Herein, based on cell imprinting and click chemistry,we have developed a cell-imprinted polydimethylsiloxane(PDMS) with aptamer functionalization(APTCIS). The atom force microscopic analysis results showed that the hierarchical structure matching well with the target cells is successfully introduced on the surface of the APT-CIS. By using the synergistic effects of hierarchical structure and aptamer affinity, the APT-CIS was successfully used for the selective cell adhesion, and 93.9% ± 0.8% of the captured cells could then be released. Thus, the APT-CIS holds promise in selective cell isolation and sorting fields.
基金
financial support from the National Key Research and Development Program (No. 2016YFA0501401)
National Natural Science Foundation of China (Nos. 21575143 and 91543201)
CAS Key Project in Frontier Science(No. QYZDY-SSW-SLH017)
Kaiguang Yang is the member of Youth Innovation Promotion Association, CAS(No. 2017222)
