哺乳动物细胞灌流培养工艺研究进展

The Research Progress of Perfusion Mammalian Cell Culture

当前生物制药领域,由于成本压力、市场需求急剧波动以及生物仿制药的竞争日益激烈,现有的生物制造技术受到诸多挑战,生物技术公司越来越倾向于开发灵活、高效的创新型生产制造工艺。灌流培养作为当前哺乳动物细胞培养的重要工艺之一,不仅可以通过不断移出副产物和添加营养物来提供有利于细胞的稳定环境,以解决蛋白质量不稳定或者表达量偏低等问题,还可以通过提高单位体积产率来优化产能利用率并提高生产效率。通过系统介绍灌流培养用于哺乳动物细胞培养的研究进展,为其进一步开发与应用提供参考。

In the current environment of biopharmaceuticals, cost pressures, rapidly fluctuating market demands and growing competition among biosimilars, existing bio-manufacturing technologies are challenged, and so that biotechnology companies are increasingly driven to develop innovative solutions for highly flexible and costeffective manufacturing.As one of the important processes in mammalian cell culture, perfusion culture has two advantages.Firstly, it can provide a stable environment favorable to cells by continuously removing by-products and adding nutrients, so that it can solve the problems of unstable protein amount or low expression level.Also, it can optimize capacity utilization and increase production efficiency by in creasing volumetric productivity.This paper systematically reviewed the progress of perfusion culture for mammalian cell culture, and it provides reference for further development and application.