急性髓系白血病患者PD-L1和MicroRNA-138-5p的表达及其临床意义

Expression and Clinical Significance of PD-L1 and MicroRNA-138-5p in Patients with Acute Myeloid Leukemia

目的:探讨PD-L1和MicroRNA-138-5p(miR-138)在急性髓系白血病(AML)患者外周血单个核细胞(PBMNCs)中的表达特点及其临床意义。方法:应用SYBR GreenⅠreal-time PCR方法分别检测20例初治AML患者、9例复发/难治AML患者和8例完全缓解患者PBMNCs中PD-L1 mRNA和miR-138的表达水平,并选择20例健康体检者外周血样本作为对照组。结果:初治AML组和复发/难治AML组患者PD-L1的表达水平均显著高于健康对照组(P<0.01),而且复发/难治AML组患者PD-L1的表达水平显著高于初治AML组患者(P<0.01);初治AML组和复发/难治AML组患者miR-138的表达水平均显著低于健康对照组(P<0.01);在20例初治AML患者中共收集了8例完全缓解期标本,完全缓解组mi R-138的表达水平高于初治AML组(P<0.05),而缓解组PD-L1的表达水平与初治AML组差异无统计学意义(P>0.05);初治AML患者中PD-L1 mRNA与mi R-138表达呈负相关(P<0.05)。结论:AML患者PBMC中PD-L1表达增加、miR-138表达下调,且两者间有相关性。

Objective:To investigate the expression and clinical significance of PD-L1 and microRNA-138-5 p in the peripheral blood mononuclear cells of patients with acute myeloid leukemia.Methods:The SYBR Green Ⅰ real-time PCR was used to detect the expression levels of PD-L1 mRNA and miR-138 in 20 cases of primary AML,9 cases of relapsed/refractory AML and 8 cases of complete remission.The samples of peripheral blood of 20 healthy peoples were used as controls.Results:The expression levels of PD-L1 in both the primary AML and the relapsed/refractory AML groups were significantly higher than those in the healthy control group(P<0.01),and the expression level of PD-L1 in the relapsed/refractory AML group was significantly higher than that in the primary AML group(P<0.01).The expression level of miR-138 in both the primary AML and the relapsed/refractory AML groups were significantly lower than that in the healthy control group(P<0.01).The 8 sampes out of 20 cases of primary AML patients achieved complete remission(CR)were collected and detected.The results showed that the expression level of miR-138 in the complete remission group was higher than that in the primary AML group(P<0.05),but the expression level of PDL1 mRNA in the CR group was not significantly different from that in the primary AML group(P>0.05).and there was a negative correlation between PD-L1 mRNA and miR-138 in primary AML patients(P<0.05).Conclusion:The expression of PD-L1 increases and the expression of miR-138 down-regulates in PBMNCs of AML patients,furthermore,both correlate each other.