复方中药制剂对急性髓系白血病细胞诱导分化与增殖的作用

Effects of a Traditinal Chinese Medicine Compound Regimen on Differentiation and Proliferation of Acute Myeloid Leukemia Cell Lines

目的:研究复方中药制剂对白血病细胞的作用及其机制。方法:用复方中药制剂处理体外培养的髓系白血病细胞株,用CCK8的方法观察中药制剂对白血病细胞增殖的影响,瑞氏染色法观察细胞形态学的变化,流式细胞术检测细胞凋亡并测定细胞表面分化抗原CD11b的表达水平。结果:与单纯的4种白血病细胞系HL-60,MOLM-13,MV4-11,AML-M5相比较,不同中药浓度处理的这4种白血病细胞增殖能力减弱(P<0.05)且其增殖抑制作用呈剂量依赖性(r=0.9236;r=0.7488;r=0.8889;r=0.8119);与单纯的HL-60和AML-M5白血病细胞系相比,药物处理的这2种白血病细胞有明显的分化形态改变;与单纯的HL-60白血病细胞系相比,IC50中药浓度处理的HL-60细胞表面抗原CD11B增加85%±7.13%;与单纯的AML-M5白血病细胞系相比,1.5μl和2μl剂量中药制剂处理的AML-M5细胞的凋亡率增加(P <0.05)。结论:复方中药制剂对白血病细胞具有抑制增值作用,其机制可能与诱导白血病细胞分化和凋亡作用相关。

Objective:To study the effect of traditional chinese medicine(TCM)compound on myeloid leukemia cells and to explore its anti-leukemic mechanism.Methods:Myeloid leukemia cell lines were cultured in vitro and treated with TCM compound.The proliferation of the leukemia cells was measured by CCK8 method.The differentiation of the leukemia cells was evaluated by using Wright’s staining method and by light microscopy,and the expression of differentiation-related surface antigens such as CD11B was measured and by flow cytometry,the apoptosis of the leukemia cells was detected by flow cytometry with using Annexin V staining.Results:Compared with untreated 4 leukemia cell lines HL-60,MOLM-13,MV4-11,AML-M5,the proliferations of 4 leukemia cells treated with different concentrations of TCM compound decreased(P<0.05),and their proliferation inhibition were in a dose-dependent manner(r = 0.9236;r = 0.7488;r = 0.8889;r = 0.8119);compared with HL-60 and AML-M5 leukemia cells,the drugtreated 2 leukemia cells displayed obvious differentiated changes;compared with untreated HL-60 leukemia cell line,the expression of surface antigen CD11B increased by 85%±7.13% in HL-60 cells treated IC50 concentration of drug;compared with untreated AML-M5 leukemia cell line,the apoptotic rate of AML-M5 treated with 1.5 and 2 μl doses of TCM compound increased.(P<0.05).Conclusion:The traditional chinese medicine compound may inhibit the proliferation of leukemia cell lines mainly by inducing leukemia cell differentiation and apoptosis.