大鼠外翻肠囊法考察对乙酰氨基酚的渗透性

Study on the permeability of Acetaminophen by rat isolated everted gut sacs

目的采用大鼠外翻肠囊模型,考察对乙酰氨基酚的渗透性。方法以大鼠外翻肠囊为模型,用HPLC法测定对乙酰氨基酚在不同肠段的透过量(Q),计算吸收速率常数(Ka)和表观渗透系数(P_(app)),评价对乙酰氨基酚在不同肠段的渗透性。结果所有组别的P_(app)值均大于高渗模型药物的P_(app)值,且不同肠段、不同给药浓度的Papp测定结果均无显著性差异。结论高、中、低浓度对乙酰氨基酚的渗透性无显著性差异;且不同肠段的渗透性无显著性差异;对乙酰氨基酚在大鼠外翻肠囊模型上表现为高渗透性。

OBJECTIVE To determine the permeability of Acetaminophen at different concentrations by everted intestinal sacs model of rat.METHODS The everted gut sacs was used to study the permeability of Acetaminophen by K)a and Papp in different segments of rat intestines(duodenum,jejunum,ileum).The samples were collected at different time and determined with HPLC.RESULTS The Papp in all groups was more than 1×10-6 cm·s-1 along without statistically significant difference in the results of different intestine segments and concentrations,which indicated that permeability of Acetaminophen in intestine was high.CONCLUSION The absorption of Acetaminophen in different intestinal segments is high.