黄芪多糖对沙门菌诱导雄性大鼠睾丸损伤的保护作用

Protective effect of Astragalus polysaccharides against the damage of testis in male rat induced by Salmonella

目的研究黄芪多糖对沙门菌诱导雄性大鼠睾丸氧化应激损伤的保护作用。方法将40只10周龄成年♂SD大鼠随机分为4组,分别进行黄芪多糖和/或沙门菌处理,处理期为7 d。试验结束后,取睾丸称重,计算绝对和相对重量。制作组织切片并观察睾丸形态与组织结构,检测生精细胞Tunel表达。分离血清,取睾丸匀浆,并测定血清和睾丸的抗氧化酶活性。结果与对照组比较,沙门菌暴露引起大鼠睾丸明显萎缩,重量显著下降,生精小管组织结构紊乱,生精细胞的Tunel表达显著增加;血清和睾丸组织的抗氧化酶活性显著降低。与单独沙门菌处理组比较,黄芪多糖+沙门菌处理组睾丸重量显著升高,生精小管的组织结构有明显改善,生精细胞的Tunel表达显著下降;血清和睾丸抗氧化酶活性显著升高;但与对照组比较,仍有较大差距。与对照组比较,单独黄芪多糖处理组的睾丸组织结构、Tunel表达和抗氧化酶活性均有一定程度提高,但差异不显著。结论沙门菌暴露诱导大鼠睾丸组织氧化应激损伤7 d后,黄芪多糖在一定程度上抑制氧化应激和生精细胞凋亡,缓解了沙门菌对大鼠睾丸组织的破坏作用。

OBJECTIVE To investigate the effect of Astragalus polysaccharides(APS)against oxidative damage of testis in a rat model induced by Salmonella and to reveal the possible mechanisms.METHODS 40 adult male SD rats of 10 week-old were randomly allocated to four groups,Salmonella and/or APS were treated orally respectively to male rats.At the end of the treatment,the absolute and relative weights of testis were weighed and calculated.Tissue sections were made and testicular morphology and structure were observed.Tunel in spermatogenic cells was detected.The antioxidant enzyme activities of serum and testis were measured after serum was separated and testicular homogenate was saved.RESULTS Compared with the control group,Salmonella exposure resulted in significant atrophy of testis,significant decrease in weight,abnormal structure of seminiferous tubules and increase in Tunel of spermatogenic cells.Salmonella exposure also resulted in significant decrease in antioxidant enzyme activity of serum and testicular tissue.Compared with the group treated with Salmonella alone,the weight of testis in APS+Salmonella group was significantly increased,the tissue structure of seminiferous tubules was significantly improved,and the expression of Tunel in seminiferous cells was significantly decreased.Serum and testis antioxidant enzyme activity were increased significantly.But there was still a big gap in the detected indices compared with the control group.The testicular tissue structure,Tunel expression and antioxidant enzyme activity in the APS treatment group were increased to some extent as compared with the control group,but the difference was not significant.CONCLUSION One-week Salmonella exposure can induce oxidative stress damage in rat testis tissue.APS can effectively alleviate the damage of Salmonella to rat testis tissue by inhibiting oxidative stress and spermatogenic cell apoptosis.