桐花树叶片的转录组分析

Transcriptome Data Analysis of Aegiceras corniculatum Leaves

为了进一步了解桐花树耐盐的分子机制,本研究采用Illumina HiSeq 2000高通量测序平台对桐花树叶片转录组进行测序,经de novo组装后获得73 721个Unigenes,进一步利用7个公共数据库(NR, NT,GO, COG, KEGG, SwissProt和InterPro)进行比对,注释了50 338个Unigenes。结果表明,有34 991个Unigenes参与到136条KEGG通路上,其中在花青素和类黄酮合成途径中的Unigenes分别有49个和176个,这些基因可能参与了桐花树应对逆境的调控过程;此外,从转录组序列中搜索到34 509个SSR位点,其中二碱基重复出现次数最多,出现频率为65.75%;从转录组的数据中预测到了2 255个转录因子,分属于58个家族。本研究结果为桐花树的基因功能分析、分子标记开发和遗传多样性研究提供一定的理论参考。

The aim of this study was to further understand the salt-resistance molecular mechanism of Aegiceras corniculatum (L.) Blanco. In this study, the transcriptome of 4. corniculaium leaf have been sequenced by Illumina HiSeq 2000. Then all the clean reads were de novo assembled, 73 721 Unigenes were yielded. After assembly, 50 338 Unigenes were annotated with 7 databases (NR, NT, GO, COG, KEGG, SwissProt and InterPro). A total of 34 991 transcripts were involved in 136 KEGG pathways, the number of Unigenes participated in pathway: anthocyanin biosynthesis and flavonoid biosynthesis were 49 and 176, respectively. These genes may play an important role in stress-responding regulation of A. corniculaium. In addition, 34 509 SSR were detected from Unigenes, in which di-nucleotide repeats most with a frequency of 65.75%. Furthermore, 2 255 transcription factors were predicted and classified into 58 families. The results of this study should be value in A. corniculaium gene function analysis, molecular marker development and genetic diversity research.