植物串联CCCH锌指蛋白RR-TZF家族研究进展及生物信息学分析

Research Progress and Bioinformatics Analysis of RR-TZF Family of Plant Tandem CCCH Zinc Finger Proteins

典型的串联CCCH型锌指结构蛋白(tandem CCCH zinc finger proteins, TZFs)含有由18个氨基酸分隔的二个串联CCCH motifs (C-X7-8-C-X5-C-X3-H、C-X5-C-X4-C-X3-H)。富含精氨酸的RR-TZF蛋白是植物特有,研究表明RR-TZF蛋白通过介导RNA的降解来调节基因的表达水平,影响植物的生长发育以及胁迫反应。本研究主要综述了植物RR-TZF蛋白在生长发育及胁迫反应中的功能及作用机制等;同时利用生物信息学手段对RR-TZF蛋白进行聚类分析,系统地分析了RR-TZF蛋白从低等植物到高等植物中的进化与保守性,并展望了未来可能的研究方向。为探究CCCH型锌指结构蛋白家族基因在作物中的具体功能及其应对非生物胁迫的分子机制研究提供了重要的理论依据。

The typical tandem CCCH zinc finger(TZF) protein contains two tandem CCCH motifs, C-X7-8-C-X5-C-X3-H and C-X5-C-X4-C-X3-H, which are spaced by 18 amino acids. Arginine-rich TZF(RR-TZF) proteins are specific in plant. Researches showed that the expression level of genes were regulated through mediating RNA degradation by RR-TZF protein, as well as affected the plant growth and stress response. This review mainly summarized the functions and reaction mechanisms of plant RR-TZF proteins during growth, development and stress responses. Besides, clustering analysis was performed on RR-TZF protein in combination with bioinformatics.The evolution and conservation of RR-TZF proteins from lower to higher plants was discussed and potential direction in future research was also proposed. The study would provide important theoretical basis for exploring the specific functions of CCCH zinc finger protein family gene in crops and revealing its molecular mechanisms in response to abiotic adversities.