miR-208作为急性心肌梗死的标志物研究

Study on miR-208 as a Marker of Acute Myocardial Infarction

目的研究miR-208在急性心肌梗死(AMI)患者中的表达水平。方法入选2015年4—12月住院和健康体检者共91例,其中包括明确诊断为AMI的患者53例(AMI组)和健康对照者38例(对照组)。收集急性心肌梗死后2小时内采血,分离血浆提取总RNA,用TaqMan~? microRNA RT-PCR试剂盒进行反转录,使用线虫microRNA cel-miR-39做内参,采用相对Cq法(ΔCq)检测血浆中miR-208表达水平。结果 AMI组血浆miR-208的中位值为1.24,对照组血浆miR-208的中位值为0.14,采用直线相关统计学分析AMI组血浆miR-208显著高于对照组(P=0.013)。ROC曲线下面积(AUC)为0.780,miR-208诊断AMI的敏感性和特异性分别为88%和68%。结论急性心肌梗死患者血浆miR-208在心梗发生后2小时内表达量显著升高,miR-208可能对于急性心肌梗死的诊断具有一定价值。

Objective To evaluate the plasma miR-208 expression level as a diagnostic biomarker for acute myocardial infarction(AMI).Methods 91 patients were enrolled from April to December 2015,including 53 patients diagnosed with AMI(AMI group)and 38 healthy controls(control group).The patients’blood was collected after AMI within 2 hours.Total RNA was extracted from separated plasma,the TaqMan■microRNA RT-PCR was used to reverse transcript cDNA.Nematodes microRNA cel-miR-39 as reference gene.The expression of miR-208 in plasma was measured by relative Cq method(ΔCq).Results The median value of plasma miR-208 in AMI group was 1.24.The median of plasma miR-208 in the control group was 0.14.Linear correlation analysis showed that the level of plasma miR-208in AMI group was significantly higher than that in control group(P=0.013).The area under the ROC curve(AUC)was 0.780,and the sensitivity and specificity of MI-208 in the diagnosis of AMI were 88%and 68%,respectively.Conclusion The expression level of plasma miR-208 in AMI group increased significantly within 2 hours after myocardial infarction,which may serve as a potention biomarker for AMI.