布鲁菌病患者血清piR-015151水平变化及其临床意义

Serum piR-015151 levels in patients with brucellosis and its clinical significance

目的探讨piR-015151在布鲁菌病(brucellosis)患者血清中的表达水平,评估其作为布鲁菌病辅助诊断指标的临床价值。方法收集2016—2017年在内蒙古医科大学附属医院或准格尔旗疾病预防控制中心确诊的73例布鲁菌病患者(布鲁菌病组)和东部战区总医院65例年龄、性别相匹配的健康人对照(对照组)血清标本及临床资料。实时荧光定量PCR (qRT-PCR)方法检测piR-015151在研究对象血清中的水平。应用受试者工作特征曲线(ROC)评估其作为布鲁菌病诊断指标的临床价值。结果 piR-015151在布鲁菌病患者血清的含量[中位数(25分位数,75分位数)]显著高于对照组[0.216 (0.069,0.426)vs 0.036 (0.021,0.070)],差异有统计学意义(U=834.5,P<0.01)。piR-015151诊断布鲁菌病的ROC曲线下面积(AUC^(ROC))为0.824 (95%CI:0.751～0.897),敏感性和特异性分别为75.3%和76.9%。结论 piR-015151在布鲁菌病患者血清中的含量显著升高,有望作为布鲁菌病辅助诊断的潜在指标。

Objective To investigate the levels of serum piR-015151 in patients with brucellosis and evaluate its clinical value in the auxiliary diagnosis of brucellosis. Methods Serum samples and clinical data from 73 brucellosis patients diagnosed in the Affiliated Hospital of Inner Mongolia Medical University or Zhungeer Qi Center for Disease Control and Prevention and 65 age- and gender-matched healthy controls in the Eastern Theater General Hospital of PLA during 2016 and 2017 were collected. Serum piR-015151 levels were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The diagnostic value of serum piR-01515 in brucellosis was evaluated by the receiver operating characteristic (ROC) curve. Results Serum piR-015151 levels (median [ P25 , P75 ]) in brucellosis patients (0.216 [0.069, 0.426]) were significantly higher than that in healthy controls (0.036 [0.021, 0.070], U = 834.5 , P <0.01). The area under the ROC curve (AUC^ROC ), sensitivity and specificity of serum piR-015151 in the diagnosis of brucellosis were 0.824 (95% CI : 0.751-0.897), 75.3% and 76.9%, respectively. Conclusion Serum piR-015151 levels in brucellosis patients increase significantly, which may be a potential marker in the auxiliary diagnosis of brucellosis.