产MUS-1型碳青霉烯酶香味类香味菌耐药性分析

Drug resistance analysis of Myroides odoratimimus producing MUS-1 carbapenemase

目的研究产MUS-1型碳青霉烯酶香味类香味菌的临床和微生物学特点。方法对徐州医科大学附属医院肿瘤科患者尿标本中分离的1株革兰阴性细菌,用Vitek 2型全自动微生物分析仪和16S RNA基因测序技术进行鉴定,PCR扩增bla_(MUS-1)基因。用肉汤稀释法检测抗菌药物的最低抑菌浓度(MIC)。结果发现1株产MUS-1型碳青霉烯酶的香味类香味菌。药敏结果显示,该细菌对大多数抗菌药物均耐药,对米诺环素、美罗培南敏感;亚胺培南的MIC为8μg/mL,判定为中介。结论携带bla_(MUS-1)基因可能是导致该株香味类香味菌对碳青霉烯类药物耐药的原因。

Objective To investigate the clinical and microbiological characteristics of Myroides odoratimimus producing MUS-1 carbapenemase. Methods A strain of gram-negative bacterium isolated from the urine sample of one patient hospitalized in the oncology department of Affiliated Hospital of Xuzhou Medical University was identified by the Vitek 2 automatic microbial analyzer and 16S RNA sequencing, and its bla MUS-1 gene was detected with PCR. The minimum inhibitory concentrations (MICs) of antimicrobial drugs were determined by the broth dilution method. Results One strain of MUS-1 carbapenemase producing Myroides odoratimimus was found. The drug susceptibility test showed that it was resistant to most of antibiotics conventionally used, but sensitive to minocycline and meropenem, the MIC of imipenem was 8 μg/mL, which was judged as intermediate. Conclusion The bla MUS-1 gene may be the cause leading Myroides odoratimimus to resistant carbapenems drugs.