双酚A多代连续暴露对秀丽隐杆线虫的毒性效应研究

A Study on the Ecotoxicity Effect of Bisphenol A on Caenorhabditis elegans by Multigenerational Exposure

为更好地阐明双酚A(BPA)多代暴露的生态毒性效应,对秀丽隐杆线虫进行了连续4个世代的低浓度暴露实验(BPA浓度为0.001~10μmol/L),评价指标包括体长、运动行为(头部摆动和身体弯曲频率)、后代数目等生理指标。研究结果表明:不同浓度的BPA多代暴露会引起秀丽隐杆线虫生理水平上的世代毒性差异。在高浓度暴露组,秀丽隐杆线虫G1代的生理指标值通常可以预测BPA暴露在子代中的响应情况;但在低浓度暴露组,G1代的生理指标值不能反映子代的响应情况,在子代中可能发生累积损伤效应或自身的进化响应。

In order to understand how bisphenol A (BPA) exposure acts on the evolutionary dynamics of populations, the model animal Caenorhabditis elegans was used to conduct the multigenerational testing. Multiple endpoints at the physiological (growth, reproduction, and moving behaviours) levels were examined by successive multigenerational exposure to low-concentration of BPA (0.001 ~ 10 μmol/L) through four generations. The results showed that changes of physiological-level effects across four generations varied in magnitude and direction depending on the exposure concentrations. As for each trait tested, the first generation response could be commonly mirrored in the subsequent generations at the highest concentration of 10 μmol/L. However, at lower concentrations, the response of parental generation was a relatively poor predictor of the effects on progeny, as acclimation or cumulative damage could occur in the subsequent generations.