旱柳转录组测序及生物学分析

Transcriptome sequencing and biological analysis of willow (Salix matsudana)

对盐敏感旱柳沿江柳和耐盐旱柳9901的杂交F_1代根部进行了RNA-Seq测序和分析,共获取了107 950条Unigene,平均长度为1 076.96 bp。通过与COG、GO等8个数据库比对,60 848个基因获得注释信息,其中38 182条Unigene在GO数据库中获得注释,24 101条Unigene在KEGG数据库中获得注释。GO和KEGG富集分析结果表明,差异表达基因主要调节核糖体代谢、植物激素信号转导等生物学功能。

In this study, RNA-Seq sequencing and analysis were performed on the roots of F 1 hybrids of salt sensitive Yanjiang Salix matsudana and salt tolerant 9901 S. matsudana , and a total of 107 950 unigenes were obtained. The average length was 1 076.96 bp. By comparing with eight databases including COG and GO, annotation informations of 60 848 genes were obtained, of which 38 182 unigenes were annotated in the GO database and 24 101 unigenes were annotated in the KEGG database. The GO and KEGG enrichment analysis results showed that differentially expressed genes regulated some biological functions, such as ribosome metabolism and phytohormone signal transduction.