武陵山区稻瘟病菌生理小种分子鉴定体系的建立

Establishment of Molecular Identification System of Magnaporthe oryzae in the Wuling Mountain Area

[目的]建立武陵山区稻瘟病菌生理小种分子鉴定体系。[方法]选用RAPD、REMAP、Rep-PCR 3种分子标记和无毒基因检测,对武陵山区稻瘟病菌生理小种文库中2013—2015年分离的220个菌株进行指纹图谱的构建和聚类分析。[结果]共统计26对引物扩增得到的3年样本中稳定出现的97条具有多态性的条带。聚类分析结果显示,在0.75的相似性水平上可将220个菌株分为12个遗传谱系,谱系10为优势谱系,不同年份的稻瘟病菌群体遗传结构存在一定差异,但并未发生明显演化。[结论]建立了新的武陵山区稻瘟病菌生理小种分子鉴定体系,为该地区抗稻瘟病菌品种的选育和布局提供参考。

[Objective]To establish molecular identification system of Magnaporthe oryzae in the Wuling Mountain Area of China.[Method]Marker system including RAPD,REMAP,Rep-PCR and Avr genes was developed for fingerprint mapping and cluster analysis of 220 M.oryzae isolates collected from the Wuling Mountain Area during 2013-2015.[Result]A total of 97 polymorphic bands amplified by 26 pairs of primers were counted.The cluster analysis data showed that 220 strains could be divided into 12 genetic lineages at the 75% similarity level,and the lineage 10 was the dominant lineage.There were some differences in the genetic structure of M.oryzae populations in different years,but no significant evolution occurred.[Conclusion]The study established a new molecular identification system for M.oryzae in the Wuling Mountain Area, and provided a reference for the breeding and distribution of the M.oryzae resistant varieties in this area.