胰蛋白酶酶解制备卵转铁蛋白源抗菌活性肽条件的研究

Preparation of Antimicrobial Peptide from Ovotransferrin with Trypsin

卵转铁蛋白具广谱抗菌性,现已成为生物医学领域研究的热点。以"两步醇析法"获得的卵转铁蛋白为研究对象,经胰蛋白酶酶解制备抗菌活性肽,优化酶解条件,并研究其对大肠杆菌的抗菌作用。结果表明,制备卵转铁蛋白抗菌肽抑菌效果最好的试验条件为:p H值8.5,温度55℃,底物浓度30 mg/mL,酶与底物浓度比1∶300(U/mg),酶解时间1.5 h,在该条件下进行酶解,得到的抗菌肽抑菌率为89.152%。

Ovotransferrin has become a hot topic in biomedical research because its broad spectrum of antibacterial activity. The ovotransferrin obtained from the "two step alcohol-precipitation" was studied. The antibacterial peptides were prepared by the enzymolysis technique-trypsin, and the hydrolysis conditions were optimized to study the antibacterial rate of E. coli. The results showed that the best experimental conditions were as follows: pH8.5, 55 ℃, substrate concentration 30 mg/mL, enzyme bottom ratio 1 ∶ 300(U/mg), enzyme hydrolysis time 1.5 h. The best bacteriostasis rate was 89.152 %.