摘要
以来源于苏云金芽孢杆菌的Bt-Cry1Ab/Ac基因为目的基因,以潮霉素Hyg为抗性标记基因,以绿色荧光蛋白基因GFP为报告基因的真菌表达载体,采用农杆菌介导真菌遗传转化(ATMT)法,将Cry1Ab/Ac基因插入白僵菌基因组中,先后用潮霉素B抗性筛选、荧光检测、真菌基因组PCR分子检测法、Bt-Cry1Ab/Ac基因检测试纸条多种方法进行转基因工程菌株鉴定,结果证实了目的基因成功插入白僵菌基因组,为白僵菌杀虫活性的深入研究奠定了基础.
Since then,the Bt-Cry1Ab/Ac gene from Bacillus thuringiensis was used as the target gene.Hygromycin (Hyg)was used as the marker gene and green fluorescent protein gene gfp as the reporter gene.The Cry1Ab/Ac gene was inserted into the genome of Beauveria bassiana by Agrobacterium tumefaciens- mediated fungal genetic transformation(ATMT).Hygromycin B resistance screening, fluorescence detection and fungal genomic PCR detection were performed successively.The results showed that the target gene was successfully inserted into Beauveria bassiana gene.The results showed that the Bt-Cry1Ab/Ac gene was successfully inserted into Beauveria bassiana.It laid a foundation for further study on insecticidal activity of Beauveria bassiana.
作者
程云清
孙贺
CHENG Yun-qing;SUN He(College of Life Science,Jilin Normal University,Siping 136000,China)
出处
《吉林师范大学学报(自然科学版)》
2019年第2期101-105,共5页
Journal of Jilin Normal University:Natural Science Edition
基金
"948"国家林业局引进项目(20150423)
吉林省产业创新专项资金项目(2016C092)