Fbxo22基因敲除小鼠模型的建立和表型研究

Generation and phenotypic analysis of Fbxo22 knockout mice

目的·分析Fbxo22基因敲除小鼠的表型,为探索FBXO22的生物学功能提供理论依据。方法·利用CRISPR-Cas9(clustered regularly interspaced short palindromic repeats-CRISPR associated protein 9)技术成功构建Fbxo22全身敲除小鼠,观察胚胎和小鼠的外观,测定其数量和质量,并分析小鼠的进食量和存活时间。结果·Fbxo22敲除小鼠胚胎期17.5/18.5 d的胚胎数量符合孟德尔遗传定律,外观未见异常,但是多数Fbxo22敲除小鼠在出生后48 h内死亡。少数存活小鼠体型偏小,进食减少,存活时间缩短。结论·FBXO22对于小鼠出生后早期存活和正常发育有重要作用。

Objective · To establish the Fbxo22 knockout mouse model and study the biological function of FBXO22. Methods · The Fbxo22 knockout mice were generated by CRISPR-Cas9 technology. The number, appearance, weight of different embryos and mice were measured. Meanwhile, the food intake and survival of Fbxo22-/-mice were analyzed. Results · Although the Fbxo22-/-embryos were present at approximately Mendelian ratios on embryonic day 17.5/18.5, most of them died within 48 hours of birth. Furthermore, those surviving Fbxo22-/-mice showed reduced body size and food intake and decreased life span. Conclusion · FBXO22 is an important, albeit not essential, protein for early postnatal survival and normal development.