摘要
Laser-activated perfluorocarb on n anodroplets are an emerging class of phase-cha nge, dual-c ontrast age nts that can be utilized in ultraso und and photoacoustic imaging. Through the ability to differe ntiate subpopulations of nano droplets via laser activatio n at differe nt wavelengths of n ear-infrared light, optically-triggered color-coded perfluorocarb on nano droplets prese nt themselves as an attractive tool for multiplexed ultrasound and photoacoustic imaging. In particular, laser-activated droplets can be used to provide quantitative spatiotemporal information regarding distinct biological targets, allowing for their potential use in a wide range of diagnostic and therapeutic applications. In the work prese nted, laser-activated color-coded perfluorocarb on nan odroplets are syn thesized to selectively resp ond to laser irradiati on at corresp on ding wavele ngths. The dyn amic ultraso und and photoacoustic signals produced by laser-activated perfluorocarbon nano droplets are evaluated in situ prior to implementation in a murine model. In vivo, these particles are used to distinguish unique particle trafficking mechanisms and are show n to provide ultraso und and photoacoustic contrast for up to 72 hours within lymphatics. Overall, the con ducted studies show that laser-activated color-coded perfluorocarbo n nano droplets are a promising agent for multiplexed ultraso und and photoacoustic imaging.
基金
D. Y. S. acknowledges fellowship funding from the National Institutes of Health (No. T32 EB007507) and the National Science Foundation Graduate Research Fellowship Program
K. A. H. acknowledges fellowship funding from the National Institutes of Health (No. T32 EB007507)
S. K. Y. acknowledges fellowship funding from the National Institutes of Health (No. F30 CA216939)
The work was supported in part by the National Institutes of Health under Grants CAI5859& EB008101 and CAI49740 as well as the Breast Cancer Research Foundation Grant (No. BCRF-17-043).
