抑郁症患者外周血miRNA-219和钙调蛋白激酶Ⅱγ亚基mRNA表达水平的临床意义

Clinical significance of miRNA-219 and calcium/calmodulin-dependent protein kinase Ⅱγ subunit mRNA levels in peripheral blood of patients with depression

目的通过检测首次发病抑郁症患者外周血中miRNA-219和钙调蛋白激酶Ⅱγ亚基mRNA(γCaMKⅡ mRNA)表达水平,分析探讨其与抑郁症之间的关系。方法纳入符合DSM-5中抑郁发作诊断标准的41例抑郁症患者(抑郁组)和31名健康对照者(对照组),采用HAMD17评估患者的抑郁症状严重程度,采集受试者空腹外周血样本,采用实时荧光定量PCR法检测外周血白细胞中miRNA-219表达水平和γCaMKⅡ mRNA表达水平,采用t检验进行组间比较;miRNA-219表达水平、γCaMKⅡ mRNA表达水平、HAMD17评分两两之间相关性采用Pearson相关分析。结果抑郁组外周血白细胞miRNA-219表达水平低于对照组(0.91±0.21与1.80±0.24,t=2.753,P=0.007 5),γCaMKⅡ mRNA表达水平高于对照组(5.55±0.57与2.16±0.20,t=4.970,P<0.01),差异均有统计学意义。并且抑郁组外周血白细胞γCaMKⅡ mRNA表达水平与HAMD17评分呈正相关(r=0.460,P=0.002 5)。结论抑郁症患者外周血miRNA-219表达水平降低,γCaMKⅡ mRNA表达水平升高,提示miRNA-219和γCaMKⅡ表达水平可能与抑郁症发病机制存在一定联系。

Objective To study the expression of miRNA-219 and γCaMK Ⅱ mRNA in the peripheral blood of the first-episode major depressive disorder (MDD) patients, and explore the relationship between these molecular level and MDD symptom. Methods Fourty-one first-episode major depressive disorder patients diagnosed with DSM-5 criteria and 31 healty volunteers were included. The blood was collected in the fasting state and the expression levels of miRNA-219 and γCaMKⅡ mRNA was detected. The patients′ severity of symptoms was assessed with HAMD17. Real-time PCR was used to detect the levels of miRNA-219 and γCaMKⅡ mRNA in peripheral blood leukocytes. The correlation between miRNA-219,γCaMK Ⅱ mRNA and HAMD17 was analyzed. Results The miRNA-219 levels of MDD group was significantly lower than that of control group (0.91±0.21 vs. 1.80±0.24, t=2.753, P=0.0075) in peripheral blood, and the γCaMK Ⅱ mRNA levels of MDD group was significantly higher than that of control group (5.55±0.57 vs. 2.16±0.20, t=4.970, P<0.01). There was the positive correlation between γCaMK Ⅱ mRNA levels and the HAMD17 score of MDD (r=0.460, P=0.0025). Conclusion The levels of miRNA-219 in peripheral blood of MDD patients decreased, and the levels of γCaMKⅡ mRNA increased, suggesting that miRNA-219 and γCaMKⅡ may be related to the pathogenesis of depression.