泽泻中三萜类成分累积与基因表达的相关性研究

Correlation study between accumulation of triterpenoids and expression of relative genes in Alisma orientale

为了探讨泽泻中三萜类成分累积与三萜类合成关键酶基因表达的相关性,该研究采用UPLC-MS/MS检测不同生长阶段泽泻块茎中8种三萜类成分泽泻醇A、24-乙酰泽泻醇A、泽泻醇B、23-乙酰泽泻醇B、23-乙酰泽泻醇C、泽泻醇F、24-乙酰泽泻醇F、泽泻醇G的含量,运用qRT-PCR技术测定三萜类合成途径中关键酶基因HMGR,FPPS的表达。通过相关性分析表明,泽泻块茎中这8种三萜类成分总增长量与HMGR,FPPS基因平均相对表达量之间均存在显著的正相关性(HMGR:r=0. 998,P<0. 01; FPPS:r=0. 957,P<0. 05)。因此,该研究初步确定了HMGR,FPPS基因调控泽泻中三萜类成分合成的功能,为泽泻中三萜类成分合成及调控机制的深入研究奠定了基础。

To research the correlation between accumulation of triterpenoids and expression of key enzymes genes in triterpenoid biosynthesis of Alisma orientale,the study utilized UPLC-MS/MS method to detect eight triterpenoids content in the tuber of A. orientale from different growth stages,including alisol A,alisol A 24 acetate,alisol B,alisol B 23 acetate,alisol C 23 acetate,alisol F,alisol F 24 acetate and alisol G,and then the Real time quantitative PCR was used to analyze the expression of key enzymes genes HMGR and FPPS in triterpenoid biosynthesis. Correlation analysis showed that there was a significant positive relation between the total growth of these eight triterpenoids and the average relative expression of HMGR and FPPS(HMGR: r = 0. 998,P<0. 01;FPPS: r = 0. 957,P<0. 05),respectively. Therefore,the study preliminarily determined that HMGR and FPPS genes could regulate the biosynthesis of triterpenoids in A. orientale,which laid a foundation for further research on the biosynthesis and regulation mechanism of triterpenoids in A. orientale.