指纹图谱、多成分定量与化学计量学相结合的厚朴药材质量评价

Quality evaluation of Magnoliae Officinalis Cortex based on combinative method of fingerprint,quantitative analysis of multicomponents and chemometrics

建立指纹图谱、多成分定量与化学计量学相结合的厚朴药材质量评价方法。采用UPLC对20批次厚朴药材进行分析,建立指纹图谱共有模式,共标定13个共有峰,通过对照品比对指认8个色谱峰,分别为紫丁香酚苷、木兰箭毒碱、木兰花碱、木兰苷B、木兰苷A、和厚朴酚、厚朴酚、辣薄荷基厚朴酚,样品相似度均在0. 90之上。在此基础上,对所有批次厚朴药材中以上8种指标性成分进行含量测定。基于指纹图谱和含量测定结果,采用聚类分析和主成分分析等化学计量学方法对不同基原厚朴药材进行区分与比较,结果表明在相同的色谱条件下,厚朴和凹叶厚朴药材种内化学指纹图谱均一,种间指纹图谱存在差异;依据以8个成分含量建立的Fisher's判别分析模型所得的判别分数可区分不同基原的厚朴样品,所建立的多组分定量分析方法可用于厚朴药材的鉴别与质量控制。

This study aims to establish a combinative method based on fingerprint,assay of multi-component and chemometrics for quality evaluation of Magnoliae Officinalis Cortex. Twenty batches of samples were determined by UPLC and a common mode of fingerprint was established. The similarities between fingerprints of 20 batches of samples were over 0. 90 and the common mode were evaluated. Eight components were identified as syringing, magnocurarine, magnoflorine, magnoloside B, magnoloside A, honokiol,magnolol,and piperitylmagnolol by comparison with reference substances and their content in samples were simultaneously determined.Based on the results,the fingerprint had good consistency between the same origin and minor diversity between the different sources.Piperitylmagnolol and peak 13 could be used as a distinction with the different sources. According to content of 8 components,Fisher discriminant analysis model was established and different source sample was classified pursuant to the discriminant fraction. It is indicated that simultaneous quantification of multi components coupled with chemometrics analysis could be a well-acceptable strategy to identify and evaluate the quality of Magnoliae Officinalis Cortex.