复发性流产患者CD4^+T细胞及调节性T细胞上T细胞免疫球蛋白和免疫受体酪氨酸抑制基序的表达

Expression of T cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain on CD4^+T cells and regulatory T cells in recurrent spontaneous abortion patients

目的探讨CD4^+T细胞及调节性T(Treg)细胞上T细胞免疫球蛋白和免疫受体酪氨酸抑制基序(T cell immunoglobulin and immunorecep tortyrosine-based inhibitory motif domain,TIGIT)与复发性流产(RSA)发病的关系。方法采用前瞻性病例-对照研究收集2017年7月—2018年8月期间在复旦大学附属妇产科医院的60例RSA患者(RSA组)和68例相同孕周正常早孕者(对照组)的外周血。采用流式细胞术检测抗凝外周血中CD4^+T细胞及Treg细胞上表面分子TIGIT的百分含量。结果CD4+T细胞在外周血中的比例组间比较差异无统计学意义,且TIGIT^+CD4^+T细胞的比例组间差异也无统计学意义(P>0.05);Treg细胞在RSA组外周血中的比例低于对照组(P=0.02);但RSA组TIGIT^+FoxP3^+CD4^+T细胞所占比例为56.41%±12.36%,高于对照组中的表达(47.82%±10.53%),差异有统计学意义(P=0.03)。结论TIGIT在RSA患者外周血Treg细胞上的表达异常升高,提示其与RSA的发生可能相关。

Objective To evaluate the correlation of T cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain(TIGIT)on peripheral CD4^+T cells and regulatory T(Treg)cells with recurrent spontaneous abortion(RSA).Methods Totally 60 RSA patients(RSA group)and 68 normal pregnant women(control group)were collected in Obstetrics and Gynecology Hospital of Fudan University during July 2017 to August 2018.Flow cytometry was used to detect the proportion of TIGIT^+T cells on CD4^+T cells and Treg cells.Results Compared with normal pregnant women,the proportion of CD4^+T and TIGIT^+CD4^+T cells in RSA patients were not different,the proportion of Treg cells was lower in RSA group(5.08%±1.94%)than in control group(3.15%±1.16%)(P=0.02),but the proportions of TIGIT^+FoxP3^+CD4^+T in RSA group(56.41%±12.36%)was higher than that in control group(47.82%±10.53%)(P=0.03).Conclusion The abnormal expression of TIGIT on Treg cells in RSA patients,may be related to the occurrence of RSA,which may be a new therapeutic target for RSA.