不同地区花生网斑病菌致病性鉴定及环境条件对致病力的影响

Identification of pathogenicity of Phoma arachidicola from different geographical location and the influence of environmental conditions on pathogenicity

为了探明不同地区的花生网斑病菌致病力并筛选快速稳定的接种方法,以来自山东、云南和辽宁的6个不同地区的花生网斑病菌为材料,用孢子悬浮液对离体叶片和花生植株进行接种,结果表明致病力最强的菌株是WB-SY(分离自辽宁省沈阳市)。最佳接种条件为:开花末期使用高浓度孢子悬浮液(10~6/mL),接种湿度保持在90%～100%之间,持续保湿36h以上,温度在25～28℃之间,接种前黑暗处理24h,接种后近紫光(波长340～380nm)处理。本文筛选出的菌株和快速稳定的接种方法,将为抗病品种的筛选奠定基础。

To identify a strain of Phoma arachidicola with high pathogenicity on peanut,and to find out a better inoculation method on peanut leaves,6 strains from different geographical location were inoculated by spore suspension to vitro leaf and plants.The 6 strains were collected from Shandong,Yunnan and Liaoning provinces.Results showed that WB-SY(from Shenyang and Liaoning)had the highest pathogenicity.The optimal conditions for pathogenicity included high concentrations of spore suspension(10^6 spores/mL),humidity between 90-100%for more than 36 h,at temperature of 25-28℃,dark treatment 24 h before inoculation then near violet light(340-380 nm)treatment at the end of peanut flowering stage.The above results were expected to establish a stable and rapid inoculation method on developing resistant varieties.