长链非编码RNA-POU3F3在胶质瘤中表达及影响预后的相关因素研究

Expression of long non-coding RNA POU3F3 in gliomas and its prognostic factors

目的:探讨长链非编码RNA-POU3F3在神经胶质瘤组织中的表达水平及对患者预后的影响,并探索可能影响脑胶质瘤患者预后的其他相关因素。方法:在胶质瘤组织和正常脑组织中采用qRT-PCR法检测POU3F3的表达并分析POU3F3水平与患者预后的关系。用Kaplan-Meier和Log-rank法分析患者5年生存率情况并进行检验。然后进行影响预后的单因素分析,最后进行多因素分析,探讨POU3F3表达水平是否为影响胶质瘤患者术后生存率的独立危险因素。结果:POU3F3在胶质瘤组织中表达量明显高于正常组织,并且与胶质瘤理级别呈正相关。POU3F3高表达组患者术后5年生存率明显下降,高水平表达的POU3F3、高病理级别以及KPS评分<80分均是影响患者术后预后的重要因素(P<0.05)。结论:高表达POU3F3是胶质瘤患者不良预后的独立危险因素,可能成为预测胶质瘤患者预后的重要指标。

Objective:To test the expression levels of long non-coding RNA POU3F3 in gliomas and its influence on the prognosis of gliomas,and to study other related factors that may influence the prognosis of gliomas.Methods:qRT-PCR was used to detect the expression of POU3F3 in glioma and normal brain tissues then to analyze the relationship between the level of POU3F3 and the prognosis of the patients.Five-year survival rates were analyzed and tested by Kaplan-Meier and Log-rank.Then univariate analysis was carried out.Finally,multivariate analysis was performed to determine whether POU3F3 expression levels was an independent risk factor for postoperative survival in glioma patients.Results:Compared with normal tissues,the expression of POU3F3 in glioma tissues was significantly higher,and was positively correlated with glioma grade.The 5 year survival rate of the patients with high expression was significantly lower than that of the patients with low expression(P<0.05).The high expression of POU3F3,with high pathological grade and KPS score<80 were the important factors influencing the prognosis of the patients(P<0.05).Conclusion:High expression levels of POU3F3 is an independent risk factor for the poor prognosis of glioma patients.POU3F3 can be served as an important index to predict the prognosis of glioma patients.