医用三氧联合经导管肝动脉化疗栓塞对肝癌患者肝功能及细胞免疫功能的影响

Effects of medical O3combined with TACE on liver function and cellular immune function in patients with hepatocellular carcinoma

目的探讨医用三氧联合经导管肝动脉化疗栓塞(TACE)术治疗对肝癌患者肝功能及细胞免疫功能的影响。方法 62例肝癌患者,分为对照组及观察组,每组各31例,对照组行TACE术治疗,观察组行医用三氧联合TACE术治疗。分别于治疗开始前及术后10 d采血行单克隆抗体标记流式细胞仪检测CD_3、CD_4、CD8、自然杀伤细胞(NK)及肝功能检查[丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、总胆红素(TBIL)]。结果与治疗前相比,观察组治疗后ALT、AST及TBIL降低,CD_3、CD_4、NK升高,CD_8下降,但差异均无统计学意义(P> 0.05);对照组治疗后ALT、AST及TBIL均显著升高,CD_3、CD_4、NK下降,CD_8升高,其中ALT、AST、TBIL、CD_4、CD_8、NK的变化差异具有统计学意义(P <0.05)。与对照组治疗后相比,观察组治疗后ALT、AST及TBIL均有下降;CD_3、CD_4、NK升高,CD_8下降,其中ALT、AST、CD_3、CD_4、CD_8的差异具有统计学意义(P <0.05)。结论医用三氧联合TACE术治疗可显著降低患者肝功能损伤的风险,增强术后细胞免疫功能。

Objective To probe the influence of medical O3 combined with transcatheter arterial chemoembolization(TACE)on liver biochemical and cellular immune function in patients with hepatocellular carcinoma.Methods Sixty-two patients with primary liver cancer were randomly divided into control group(31 cases with therapy of TACE alone)and observation group(31 cases treated with medical O3 combined with TACE).Before and 10 days after treatment,we used monoclonal antibody marker flow-cytometry to detect the cytokines(CD3,CD4,CD8,NK)and liver function(ALT,AST,TBIL)in all patients.Results Compared with prior treatment,ALT,AST and TBIL levels reduced in the observation group after treatment,while CD3,CD4,NK levels elevated and CD8 decreased.But the differences were not statistically significant(P>0.05).Post treatment in the control group,ALT,AST and TBIL levels were significantly increased,while CD3,CD4 and NK levels were decreased but CD8 increased.The differences of ALT,AST,TBIL,CD4,CD8 and NK were statistically significant respectively(P<0.05).Moreover,the levels of ALT,AST,TBIL and CD8 decreased and CD3,CD4 and NK increased in the observation group post treatment compared with those of the control group.The differences of ALT,AST,CD3,CD4 and CD8 levels between the two groups were statistically significant(P<0.05).Conclusion The combination of medical O3 and TACE can significantly reduce the risk of liver function injury and enhance the postoperative cellular immune function.