高浓度反全式维甲酸对人舌鳞癌细胞Cal27增殖的影响

The effect of all-trans-retinoic acid on the proliferation of human tongue squamous cell carcinoma Cal27

目的探讨较高浓度反全式维甲酸对口腔鳞癌Cal27细胞系增殖、凋亡、周期、迁移等生物学过程的影响,为其在口腔鳞癌临床治疗中的作用提供理论支持。方法用不同浓度反全式维甲酸对口腔舌鳞癌Cal27细胞进行刺激,分别用CCK-8法检测增殖,流式检测细胞凋亡、细胞周期,细胞划痕法检测细胞迁移能力。结果反全式维甲酸对舌鳞癌Cal27细胞进行处理,发现不同浓度反全式维甲酸均可抑制Cal27细胞增殖,尤其在100μmol/L时,细胞增殖明显变慢,几乎处于增殖停滞状态,而相同浓度反全式维甲酸对正常皮肤成纤维细胞的增殖未见有显著影响。在迁移实验中,3个浓度梯度均能减慢细胞迁移,60μmol/L与100μmol/L 2个浓度对迁移影响较大,特别是在100μmol/L时,细胞24 h几乎未有任何迁移。凋亡结果显示,不同浓度作用细胞时,均存在凋亡增加的现象,但不同药物浓度之间凋亡细胞数量未见明显差异(P> 0.05)。结论反全式维甲酸可通过增加凋亡,改变细胞周期,进而抑制舌鳞癌细胞的活性,减慢细胞增殖,抑制程度随浓度增高而增强。

Objective To investigate the effects of all-trans-retinoic acid with higher concentration on the biological process of oral squamous carcinoma Cal27,such as proliferation,apoptosis,cell cycle and migration,and to provide theoretical support for clinical treatment of this disease. Method Cal27 cells were incubated with all-trans-retinoic acid in different concentration. Proliferation rate was detected by cck-8 assay. Cell apoptosis and cell cycle were detected with FCM. Cell migration rate was calculated with cell scratching assay. Result The cal27 cells were incubated with all-trans-retinoic acid and we found that all-trans-retinoic acid with different concentration could all inhibit the proliferation of cal27,especially the 100 μmmol,which greatly reduce the proliferative rate of cal27 cells. The cell scraching assay shows that all three concentrations could slow down the migration rate of Cal27,the 60 μmmol and 100 μmmol have strong effect on the migration rate of Cal27. The cells were all experience stronger apoptosis when treated with all-trans-retinoic acid,but there is no statistical differences between different groups. Conclusion all-trans-retinoic acid could increase apoptosis,throngh decreasing cell activity and slowing down Cal27 proliferation.