PI3K-AKT-mT0R 信号通路在肝癌组织中的表达及意义

Expression and significance of PI3K-AKT-mTOR signaling pathway in hepatocellular carcinoma

目的探讨PI3K-AKT-mTOR信号通路在肝癌组织中的表达及临床意义。方法收集原发性肝癌组织标本及癌旁组织标本70例.采用免疫组化法检测肝癌组织和癌旁组织中的p-AKT及p-mTOR阳性表达情况,并采用WB法检测肝癌组织和癌旁组织中的p-AKT及p-mTOR蛋白相对表达水平情况,分析肝癌组织中p-AKT及p-mTOR蛋白表达量与临床病理特征的关系及两者在肝癌组织中的相关性结果免疫组化结果显示,肝癌组织中p-AKT及p-mTOR阳性表达率分别为71.43%、77.14%,均明显高于癌旁组织的28.57%、62.86%(P < 0.05 )。WB法检测结果显示,肝癌组织中p-AKT及p-mTOR表达水平分别为(0.62 ±0.11 )、(0.55±0.10 ),明显高于癌旁组织的(023 ±0.07 )、( 0.33 ± 0.09 )(P < 0.05 )。中低分化、有淋巴结转移、III+IV期的肝癌组织中p-AKT及p-mTOR表达水平明显高于高分化、无淋巴结转移、I+II期的肝癌组织(P < 0.05)。肝癌组织中p-AKT与p-mTOR表达水平呈正相关(r = 0.564,P = 0.000 )。结论PI3K-AKT-mTOR信号通路在肝癌组织中存在过度表达,这可能是肝癌发生、进展及转移的一个机制,可能是肝癌防治的一个新靶点。

Objective To investigate the expression and clinical significance of PI3K-AKT-mTOR signaling pathway in hepatocellular carcinoma. Methods 70 specimens of primary hepatocellular carcinoma and adjacent tissues were collected. The positive expression of p-AKT and p-mTOR in hepatocellular carcinoma and adjacent tissues were detected by immunohistochemical method. The relative expression of p-AKT and p-mTOR protein in hepatocellular carcinoma and adjacent tissues were detected by WB method. The relationship between the expression of p-AKT and p-mTOR protein and clinicopathological characteristics were analyzed, and the correlation between them in hepatocellular carcinoma. Results Immunohistochemical results showed that the positive rates of p-AKT and p-mTOR in HCC tissues were respectively 71.43% and 77.14%, which were significantly higher than those in adjacent tissues (28.57%, 62.86%)(P<0.05). WB assay showed that the expression levels of p-AKT and p-mTOR in hepatocellular carcinoma were (0.62±0.11) and (0.55±0.10), significantly higher than those in adjacent tissues [(0.23±0.07),(0.33±0.09)](P<0.05). The expression levels of p-AKT and p-mTOR in moderately and poorly differentiated HCC, HCC with lymph node metastasis, and stage III+IV HCC were significantly higher than those in highly differentiated HCC, HCC without lymph node metastasis, and stage I+II HCC (P<0.05). There was a positive correlation between p-AKT and p-mTOR expression in hepatocellular carcinoma (r=0.564, P=0.000). Conclusion PI3K-AKT-mTOR signaling pathway is over-expressed in hepatocellular carcinoma, which may be a mechanism for the occurrence, progression, and metastasis of hepatocellular carcinoma and a new target for the prevention and treatment of hepatocellular carcinoma.