期刊文献+

微小RNA-542-3p在人脑胶质瘤中的表达及其对细胞增殖迁移能力的影响 被引量:9

Expression of microRNA-542-3p in human glioma and its effect on cell proliferation and migration
原文传递
导出
摘要 目的观察微小RNA(miRNA,miR)-542-3p在脑胶质瘤患者肿瘤组织中的表达及其对肿瘤细胞增殖、迁移的影响。方法采用实时荧光定量聚合酶链反应(FQ-PCR)检测33例脑胶质瘤患者肿瘤组织及29例颅脑外伤患者正常脑组织中miR-542-3p的表达水平;合成miR-542-3p mimics及无关序列(miR-542-3p NC),并采用Lipofectamine 2000分别将miR-542-3p mimics和miR-542-3p NC转染至人胶质瘤U87细胞株;分别进行培养后,采用细胞计数试剂盒(CCK-8)检测细胞的增殖能力;划痕实验检测细胞的迁移能力;Transwell实验检测细胞的侵袭能力,评价miR-542-3p在胶质瘤细胞U87中的生物学功能。结果微小RNA miR-542-3p在脑胶质瘤组织及正常脑组织中的相对表达量分别为0.74±0.06和1.06±0.09,胶质瘤组织中miR-542-3p的相对表达水平显著低于正常脑组织(t=3.03,P<0.01)。在人胶质瘤细胞株U87中分别转染miR-542-3p mimics和无关序列miR-542-3p NC后,转染miR-542-3mimics对U87细胞增殖能力无明显影响(t=0.236,P>0.05)。但U87细胞中转染miR-542-3p mimics后细胞的迁移能力和侵袭能力明显增强。结论miR-542-3p在人胶质瘤细胞中表达水平减低,并在胶质瘤细胞中发挥抑制细胞迁移和侵袭。 Objective To evaluate microRNA (miRNA, miR)-542-3p expression in glioma and its effect on cell proliferation and migration. Methods The expression of miR-542-3p in tumor tissues of 33 patients with glioma and 29 normal brain tissues of patients with traumatic brain injury was examined by real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) was used to detect. Synthesize miR-542-3p mimics and unrelated sequences (miR-542-3p NC) were transfect into human glioma cells (U87) by Lipofectamine 2000, respectively. Cell proliferation, migration and invasion ability was examined by cell counting kit-8 (CCK-8), wound healing and Transwell assay respectively. Results The relative expression levels of miR-542-3p in glioma and normal brain tissues were 0.74±0.06 and 1.06±0.09, respectively. The relative expression levels of miR-542-3p in glioma tissues were significantly lower than those in normal brain tissues (t=3.03, P<0.01). Mi-542-3p mimics and microRNAs-542-3p NC were transfected into human glioma cell line U87, and had no significant effect on the proliferation of U87 cells (t=0.236, P>0.05wever, after transfection of miR-542-3p mimics into U87 cells, the migration and invasion ability of cells increased significantly. Conclusion MiR-542-3p expression in human glioma cells is decreased, and inhibite cell migration and invasion in glioma cells.
作者 杨彬 于晶 王亮 陈镭 Yang Bin;Yu Jing;Wang Liang;Chen Lei(Department of Neurosurgery, 5th Central Hospital of Tianjin, Tianjin 300450, China)
出处 《中华实验外科杂志》 CAS CSCD 北大核心 2019年第4期759-761,共3页 Chinese Journal of Experimental Surgery
关键词 胶质瘤 微小RNA-542-3p 增殖 侵袭 转移 Glioma MicroRNA-542-3p Proliferation Invasion Metastasis
  • 相关文献

同被引文献93

引证文献9

二级引证文献19

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部