2型糖尿病并发阿尔茨海默病患者外周血淋巴细胞蛋白磷酸酯酶-2A的变化

Changes of protein phosphotase-2A level in lymphocytes of type 2 diabetes mellitus accompained by Alzheimer′s disease

探讨2型糖尿病(2型DM)并发阿尔茨海默病(AD)患者外周血淋巴细胞蛋白磷酸酯酶-2A(PP-2A)水平的变化情况。方法研究对象为2016年7月至2017年10月在上海市第六人民医院东院神经内科和淄博市第一人民医院神经内科就诊的患者,共57例,分为AD组(n=20)、2型DM组(n=20)和2型DM并发AD组(n=17),选取同期健康体检者(n=23)为对照组。用^(32)P放射性标记法分别检测所有患者及对照组外周血淋巴细胞PP-2A的活性,并用免疫印迹法检测血淋巴细胞PP-2A的表达。结果与正常对照组淋巴细胞PP-2A活性及表达(0.99±0.06,1.02±0.09)比较,2型DM组(0.87±0.11,0.91±0.10)、AD组(0.76±0.13,0.86±0.11)、2型DM并发AD组(0.62±0.72,0.71±0.09)PP-2A活性及表达均降低(F=5.27,7.74;均P<0.05)。2型DM并发AD组PP-2A活性及表达较2型DM组降低(LSD-t=7.40,5.85;均P<0.05),较AD组亦降低(LSD-t=4.56,4.57;均P<0.05); AD组较2型DM组PP-2A活性降低(LSD-t=2.84,P<0.05),但其表达降低差异无统计学意义(LSD-t=1.28,P>0.05)。结论 2型糖尿病并发阿尔茨海默病血淋巴细胞PP-2A的活性及表达降低,提示此蛋白酯酶对此类疾病有潜在的诊疗价值。

Objective To explore the alterations of protein phosphotase-2A (PP-2A) level in lymphocytes in the patients with type 2 di abetes mellitus (type 2 DM) accompained by Alzheimer′s disease(AD). Methods From July 2016 to October 2017, 57 patients in Neurology department of east section, Shanghai Sixth People′s Hospital East and the First People′s Hospital of Zibo were enrolled. All patients were divided into AD group ( n =20), type 2 DM ( n =20) and type 2 DM accompained by AD group ( n =17). Healthy subjects ( n =23) of the east section of Shanghai Sixth People′s Hospital for the same period were selected as the control group. The activity of PP-2A was determined by 32 P-labelling, and the PP-2A expression of each group was measured by western- blotting. Results Compared with control group(0.99±0.06,1.02±0.09), the activity and the expression of PP-2A were decreased in type 2 DM group (0.87±0.11,0.91±0.10), AD group(0.76±0.13, 0.86±0.11) and type 2 DM accompained by AD group (0.62±0.72,0.71±0.09), the differences were statistically significant ( F =5.27,7.74,all P <0.05). Furthermore, the activity and the expression of PP-2A in type 2 DM accompained by AD group were lower than those in type 2 DM group (LSD- t =7.40,5.85, P <0.05) and AD group (LSD- t =4.56, 4.57, P <0.05). The activity of PP-2A in AD group was lower than that in type 2 DM group (LSD- t =2.84, P <0.05), but the expression of PP-2A between the two groups was not statistically significant (LSD- t =1.28, P >0.05). Conclusion Lower activity and expression of PP-2A level in lymphocytes in type 2 DM accompained by AD patients, implying a possible value for diagnosis and treatment.