HPLC法同时测定不同产地甘西鼠尾草及丹参中8种主要成分的含量

Simultaneous determination of 8 constituents in Salvia przewalskii Maxim and Radix Salviae miltiorrhizae from different areas by HPLC

目的建立同时测定甘西鼠尾草及丹参中丹参素钠、迷迭香酸、紫草酸、丹酚酸B、二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA含量的HPLC法,对比不同产地甘西鼠尾草及丹参中8种成分的含量。方法采用SunFire C_(18)(250 mm×4.6 mm,5μm)色谱柱。流动相为乙腈(A)-0.8 mL·L^(-1)甲酸超纯水溶液(B),梯度洗脱(0～19 min,20%A～60%A;19～49 min,60%A～81%A;49～53 min,81%A～91%A;53～57 min,95%A)。流速:1.0 mL·min^(-1);检测波长:270 nm;柱温:26℃;进样量:2μL。结果质量浓度与峰面积线性关系良好(r>0.971 3),精密度、重复性及回收率均符合要求。在50 min内甘西鼠尾草及丹参中8种成分均可实现完全分离。结论青海不同产地甘西鼠尾草较其他产地甘西鼠尾草在脂溶性成分和水溶性成分上均有较好的优势,与丹参相比主要的差别在于丹酚酸B和迷迭香酸的含量,丹酚酸B的含量较丹参样品低,其余4种脂溶性成分均优于丹参样品。

Objective To establish an HPLC method for the simultaneous determination of 8 compounds including sodium danshensu ,rosmarinic acid,lithospermic acid,salvianolic acid B, dihydrotanshinquinone-Ⅰ and cryptotanshinone,tanshinone Ⅰ and tanshinone ⅡA in Salvia przewalskii Maxim and Radix Salviae miltiorrhizae collected from different origins,and to compare the contents. Methods The HPLC separation was performed on an SunFire C18 (250 mm×4.6 mm,5 μm) column.The mobile phase A was acetonitrile ,and the mobile phase B was acetonitrile-0.8 mL·L^-1 formic acid.The separation was achieved using the following gradient program:(0-19 min,20%A-60%A;19-49 min,60%A-81%A;49-53 min,81%A- 91%A;53-57 min,95%A),at a flow rate of1.0 mL·min^-1 . The column temperature was controlled at 26 ℃,the UV detection wavelength was 270 nm,and the injection volume was 2 μL. Results The correlation coefficients were more than 0.971 3.Precision,repeatability and recovery met the requirements.The baseline separation was obtained within 50 min for the 8 compounds. Conclusion Compared with other areas, Salvia przewalskii Maxim produced from Qinghai Province has a better advantage in terms of liposoluble and hydrosoluble constituents.Meanwhile,the main difference between Salvia przewalskii Maxim and Radix Salvia miltiorrhiza is the content of salvianolic acid B and rosmarinic acid.The content of salvianolic acid B in Salvia przewalskii Maxim is lower,but the other 4 contents are higher than Radix Salvia miltiorrhiza.