NAUTOTM自养脱氮工艺接种启动及稳定运行控制

Start-up by Inoculation and Stable Operation Control of NAUTOTM Autotrophic Denitrification Process

基于MBBR开发了一种新型全程自养脱氮工艺——NAUTO^TM,采用其处理污泥厌氧消化脱水液,考察了启动和稳定运行效果。通过接种CANON悬浮载体来缩短NAUTO^TM工艺的启动时间。在接种率为10%的情况下,运行84d后对NH4^+-N的去除率即可达83.40%,总氮去除负荷超过0.90kg/(m^3·d)。系统稳定运行超过300d,出水NH4^+-N浓度低于30mg/L,氨氮和TN去除率分别达到95.06%和89.71%,TN去除负荷最高可达1.21kg/(m^3·d)。对悬浮载体的高通量测序结果显示,NAUTO^TM工艺启动成功后,氨氧化菌(AOB)和厌氧氨氧化菌(AnAOB)都是系统中的优势菌种,稳定运行阶段丰度分别达到16.80%和23.17%,而主要干扰菌群亚硝酸盐氧化菌(NOB)和反硝化菌(DNB)被成功抑制,反硝化菌丰度仅为3.66%,几乎未检测出NOB。NAUTO^TM工艺启动时间短、运行负荷高、运行控制稳定,适合于自养脱氮的工程应用。

Based on MBBR process, a new type of autotrophic denitrification process-NAUTO^TM was developed. The start-up and stable operation of NAUTO^TM used for the treatment of reject water was introduced. The start-up time of NAUTO^TM process was shortened by inoculating CANON suspension carrier. The removal rate of NH4^+- N reached to 83. 40% and the TN removal load exceeded 0. 90 kg/(m^3·d) within 84 days after inoculated with only 10% seeding carriers. More than 300 days of stable operation, the concentration of NH4^+- N in effluent was below 30 mg/L, the removal rates of ammonia nitrogen and TN were 95. 06% and 89. 71%, respectively, and the maximum removal load of TN was 1.21 kg/(m^3·d). The result of high-throughput sequencing showed that after the successful start-up of NAUTO^TM process, ammonia oxidizing bacteria ( AOB ) and anaerobic ammonia oxidizing bacteria (AnAOB) became the dominant bacterium in the system, and the abundanee reached to 16. 80% and 23. 17% respectively in the stable operation stage. While the main interfering bacterium, nitrite oxidizing bacteria ( NOB ) and denitrification bacteria ( DNB ), were successfully inhibited. The abundance of denitrification bacteria was only 3. 66%, and almost no NOB was detected. NAUTO^TM process has the advantages of short start-up time, high nitrogen removal load and stable operation control, which is suitable for the engineering application.