摘要
本实验选用30份玉米样品,通过酶联免疫吸附(ELISA)法和高效液相色谱-质谱联用(HPLC-MS)法对玉米中黄曲霉毒素B1含量(AFB1)进行测定比较,得出两种方法的差异性和相关性。其中,使用HPLC-MS外标法定量时,设计两组标准曲线,分别是玉米基质液加标组曲线和纯标组曲线,得到的结果再与ELISA比较。结果显示:用ELISA筛选出的阳性样品,用HPLC-MS法确证亦为阳性,判定结果一致,但具体检出量存在差异。当样品中AFB1含量为2~6μg/kg时,测定结果 HPLC-MS(纯标组)<ELISA<HPLC-MS(玉米基质液加标组),且HPLC-MS(玉米基质液加标组)测定值约为ELISA和HPLC-MS(纯标组)的2~13倍;当样品中AFB1含量为11~50μg/kg时,ELISA<HPLC-MS (纯标组)≈HPLC-MS (玉米基质液加标组)。当样品中AFB1含量<61.78μg/kg时,测定结果HPLC-MS(玉米基质液加标组)≈HPLC-MS(纯标组)+3.6μg/kg。研究表明,ELISA操作简单快速,准确度较高,适用于玉米中AFB1的快速大量筛选,HPLC-MS耗时长,成本高,更适用于经ELISA初筛后呈阳性样品的确证及AFB1的精确定量。
Thirty maize samples were selected in this experiment.AFB1 in maize was detected and compared by ELISA and HPLC-MS to get the differences and correlations between the two methods.The results showed that positive sample screened by ELISA were comfirmed by HPLC-MS,but there were differences in their detectable amount.The result was HPLC-MS(pure mark group)<ELISA<HPLC-MS(maize substrate liquid add mark group)when content aflatoxin B1 in sample was from 2 to 6μg/kg,and the result of HPLC-MS(maize substrate liquid add mark group)≈ELISA or HPLC-MS(pure mark group)multiplied by 2 to 13.The result was ELISA<HPLC-MS(pure mark group)≈HPLC-MS(maize substrate liquid add mark group)when content aflatoxin B1 in sample was from 11 to 50μg/kg.And the result was HPLC-MS(pure mark group)≈HPLC-MS(maize substrate liquid add mark group)+3.6μg/kg whose content aflatoxin B1 in sample lower than 61.78μg/kg.The results suggested that ELISA was a simple and rapid method with high accuracy,and it was suitable for fast screening of aflatoxin B1 in large number of maize samples.While HPLC-MS method takes longer and costs higher to detect,so it was more suitable for the confirmation of positive sample preliminarily screened by ELISA,and the accurate quantitative of aflatoxin B1 content.
作者
陈琛
王炳彦
钟兴文
杨旭艳
马丹
戚敏
孙照程
陈莹
孔凡虎
华雪妃
章雨竹
陶琳丽
张曦
CHEN Chen;WANG Bingyan;ZHONG Xingwen;YANG Xuyan;MA Dan;QI Min;SUN Zhaocheng;CHEN Ying;KONG Fanhu;HUA Xuefei;ZHANG Yuzhu;TAO Linli;ZHANG Xi(Faculty of Animal Science and Technology,Yunnan Key Laboratory of Animal Nutrition and Feed Science,Yunnan Agricultural University,Kunming,Yunnan Province 650201,China;Animal Husbandry and Veterinary Station of Xinqiao Town in Mouding County Chuxiong Prefecture of Yunnan Province,Mouding,Yunnan Province 675501,China;Animal Health Supervision Institute of Dali Bai Autonomous Prefecture,Dali,Yunnan Province 671000,China;Veterinary Drug and Feed Monitoring Station of Yunnan Province,Kunming,Yunnan Province 650201,China)
出处
《中国饲料》
北大核心
2019年第9期87-92,共6页
China Feed
基金
云南省重大科技专项项目"高原优质肉鸡产业工程化技术研发集成与示范推广"(2016ZA008号)
云南省建立农科教相结合新型农业社会化服务体系试点项目"高原优质地方鸡"(2014NG006号)
云南省现代农业产业技术体系建设项目"云南省禽蛋产业技术体系建设"(2017KJTX0017)