微流控芯片上细胞样本低温保存的研究进展

Research progress in on-chip cryopreservation of living cells

随着分子、细胞以及微组织、器官模型等逐渐转移到微流控芯片上,微流控芯片的平台性价值在生物医学领域日益凸显。发展芯片上细胞样本的低温保存技术不仅是实现上述价值的重要条件,也是对传统细胞样本低温保存的有益创新。对微流控芯片上细胞样本的低温保存技术进行了综述,包括慢速冷冻保存及其升降温过程的控制、超快速降温玻璃化保存的理论分析与验证,并在此基础上进一步介绍了芯片上细胞样本的常温保存,最后对该领域进展中存在的动力与需要解决的问题作了简要分析与展望,以期为相关研究提供参考。

With the gradual integration of molecules, cells and micro tissues, organ models into microfluidic chips, the platform value of microfluidic chips is increasingly prominent in biomedical field. The development of cryopreservation of cell samples on chip is not only the essential condition for realizing this value, but a beneficial innovation for traditional cryopreservation of cell samples. In this paper, the cryopreservation techniques of cell samples on microfluidic chips were reviewed, including slow freezing of cell samples on chips and temperature control in this process, theoretical analysis and verification of vitrification of cell samples on chips. Furthermore, preservation of cell samples on chips at non-cryogenic temperature was introduced. Finally, the dynamics and problems in on-chip cryopreservation of cell samples were briefly analyzed to provide reference for relative researches.