血清miRNA-370与颈动脉粥样硬化微栓子形成间的关系研究

Relationship between serum miR-370 level and formation of carotid atherosclerosis micro embolus

目的探讨颈动脉粥样硬化微栓子(MES)的形成与患者血清微小RNA-370(miR-370)表达水平间的关系。方法从心内超声科确诊的高血压合并颈动脉粥样硬化患者中筛选出不稳定性斑块患者135例,应用经颅彩色多普勒检查鉴定出MES阳性73例(MES阳性组)和MES阴性62例(MES阴性)。同时,选取60名健康者作为对照组。采用实时定量PCR技术检测所有研究对象血清miR-370的表达水平。结果 MES阳性和阴性组患者血清中miR-370的表达量均高于对照组,且MES阳性组更显著,差异均有统计学意义(P<0.01)。根据上调的中位倍数(3.05倍)分为miR-370高表达(>3.05倍,68例)和低表达(≤3.05倍,67例)。MES阳性组中miR-370高表达的患者显著高于MES阴性组(61.6%vs 37.1%,P<0.01)。Spearman相关性分析显示,血清miR-370表达水平与低密度脂蛋白、总胆固醇、甘油三酯及同型半光氨酸水平呈正相关(P<0.05或0.01)。结论血清miR-370表达水平与颈动脉粥样硬化MES的形成有关,或可作为分子标记物监测斑块的发生和发展。

Objective To investigate the relationship between serum miR-370 level and formation of carotid atherosclerosis micro embolus (MES).Methods A total of 135 cases with unstable plaque were selected from the patients diagnosed with hypertension complicated with carotid atherosclerosis by the Department of Intracardiac Ultrasound.There were 73 cases with MES positive (MES Positive Group) and 62 cases with MES negative (MES Negative Group) identified in transcranial color Doppler examination.At the same time,60 healthy persons were selected as the Control Group.The MiR-370 expression in all serum samples was detected by using real-time PCR technique.Results The expression of miR-370 in MES Positive and Negative Group was higher than the Control Group,and the MES Positive Group showed a more significant increase,and the difference was statistically significant (P<0.01).According to the upregulated "multiples of the median"(3.05),it was divided into miR-370 high expression (>3.05,68 cases) and miR-370 low expression (≤3.05,67 cases).The number of patients with miR-370 high expression in MES Positive Group was greater than that in MES Negative Group (61.6% vs 37.1%,P<0.01). The Spearman correlation analysis showed that serum miR-370 level was positively correlated with LDL,total cholesterol, triglycerides and homophenine level (P<0.01).Conclusion Serum miR-370 level in patients with carotid atherosclerosis is significantly relevant to the formation of MES and may represent a novel biomarker for monitoring the occurrence and development of plaque.