液相芯片技术检测儿童急性呼吸道感染病原体的研究

Study on detection of acute respiratory infection pathogen in children by using liquid-phase microarray

目的探讨液相芯片技术快速检测呼吸道病原体的可行性,为重大传染病原体核酸快速应急检测平台的建立提供实验依据。方法采用xTAG~?Respiratory Viral Panel Fast v2试剂盒对74例咽拭子临床标本进行检测,结果与实时荧光定量PCR(RT-PCR)进行比较。结果 74例咽拭子临床标本经液相芯片技术与RT-PCR检测,阳性率分别为77.0%和68.9%,差异有统计学意义(P<0.05);其中呼吸道合胞病毒、鼻病毒、肠道病毒、甲型H1N1流感病毒、腺病毒及人博卡病毒检出率差异均有统计学意义(P<0.05)。结论液相芯片技术可以较准确地鉴定呼吸道病原体和检测混合感染的临床标本,具有快速、高通量等优势,可应用于呼吸道病原体的快速应急检测。

Objective To explore the feasibility of rapid detection of respiratory pathogens with liquid-phase microarray technology and provide experimental basis for the establishment of rapid emergency detection platform for nucleic acid of major infectious pathogens.Methods The 74 cases of pharyngeal swab clinical specimens were detected with xTAG■Respiratory Viral Panel Fast v2 kit and fluorescence quantitative RT-PCR.Results The positive rates of 74 clinical samples of pharyngeal swab were 77.0%and 68.9%respectively by using liquid-phase microarray and RT-PCR,and the differences were statistically significant(P<0.05).Among them,respiratory syncytial virus,Rhinovirus,Enterovirus,H1N1,Adenovirus and Human Bocavirus had statistically significant differences in the detection rates(P<0.05).Conclusion Liquid-phase microarray technology can accurately identify respiratory pathogens and detect clinical samples of mixed infection,which has advantages of rapid and high throughput.This method can be applied to rapid emergency detection of respiratory pathogens.