慢性心理应激导致小鼠γδT细胞数目减少

Restraint stress leads to reduced number of γδT cells in mice

目的:研究慢性心理应激以及起主要作用的应激激素对于γδT细胞的影响。方法:采用连续5次16 h/次束缚模型诱导慢性心理应激,取小鼠胸腺、脾脏进行称重和白细胞计数,取肠系膜淋巴结进行白细胞计数,流式细胞术检测CD3+γδTCR+细胞的百分比,检测糖皮质激素受体抑制剂和儿茶酚胺β肾上腺素能受体抑制剂的可能逆转作用,分析体外糖皮质激素对纯化的γδT细胞凋亡的影响。结果:连续5次16 h/次束缚导致小鼠免疫器官的重量和细胞数量减少,γδT细胞数量减少;阻断糖皮质激素的外周作用可逆转γδT细胞数量减少,糖皮质激素可在体外直接诱导纯化的γδT细胞凋亡。结论:慢性心理应激是通过糖皮质激素的刺激作用导致γδT细胞的凋亡和减少。

Objective:To explore how chronic psychological stress might affectγδT cells.Methods:Five 16 h restraint sessions were used to induce chronic psychological stress.The organ weight and cell numbers of the mouse thymus and spleen were measured.The cell numbers of mesenteric lymph nodes were also measured.Then single-cell suspensions were subjected to flow cytometry analysis of the percentages of CD3^+γδTCR^+cells.Glucocorticoid receptor inhibitor and beta-adrenergic receptor inhibitor were used to explore the possible reversal.Whether glucocorticoid directly induced apoptosis of purifiedγδT cells in vitro was also analyzed.Results:Here,we report that the reduction ofγδT cells upon chronic psychological stress occurs in the thymus,spleen,and mesenteric lymph nodes,which could be reversed by blockade of glucocorticoid peripheral action.Furthermore,glucocorticoid directly induces apoptosis of purifiedγδT cells in vitro,which is associated with augmented apoptosis ofγδT cells in the spleen of stressed mice.Conclusion:Chronic psychological stress leads to apoptotic loss ofγδT cells through glucocorticoid.