HPV检测方法及微流控芯片技术的应用

HPV detection method and the application of microfluidic chip technology in it

人乳头瘤病毒(HPV)引起的黏膜感染是宫颈癌的主要致病因素,鉴于病毒流行广泛,感染反复和潜伏时间较长等特点,亟待发展新的、低成本的技术和方法,进行有效的人口水平筛查和监测。近些年,随着检测宫颈癌和HPV-DNA的方法与技术的发展,出现了集成核酸提取、扩增和检测一体化的微流控芯片技术,该技术由于具有微型化、成本低廉、信号稳定、灵敏度高和特异度强等优势而被视为宫颈癌和HPV-DNA的理想的筛查平台。微流控芯片技术的出现为低资源配置下的癌症筛选、诊断、疫苗接种和术后反应的监测提供了理论依据和技术支持。

Mucosal infection caused by the human papillomavirus (HPV) is the major pathogenic factor of cervical cancer.In view of the wide spread of the virus,repeated infection and long latency,it is urgent to develop new and low-cost technologies and methods for effective population screening and monitoring.In recent years,with the development of methods and technologies for detecting cervical cancer and HPV-DNA,microfluidic chip technology integrated with nucleic acid extraction,amplification and detection has emerged.Because of its advantages of miniaturization,low cost,stable signal,high sensitivity and specificity,this technology is considered as an ideal screening platform for cervical cancer and HPV-DNA.The emergence of microfluidic chip technology provides theoretical basis and technical support for cancer screening,diagnosis,vaccination and monitoring of post-operative response under low resource allocation.