多种方法联合检测附红细胞体的应用

Application of multiple methods for Eperythrozoon determination

目的通过多种方法联合检测以提高人附红细胞体检测的特异性。方法选取223例血液病患者,分别用瑞氏-吉姆萨染色和吖啶橙荧光染色检测附红细胞体,比较2种方法的附红细胞体检出率。对2种方法检测结果均阳性的标本进行聚合酶链反应(PCR)检测,并对PCR检测结果阳性的标本进行基因测序和GenBank比对分析。结果瑞氏-吉姆萨染色附红细胞体的检出率为46.19%,吖啶橙荧光染色附红细胞体的检出率为28.25%。2种方法检测结果均阳性的40例标本中有10例PCR检测结果阳性,取其中1例条带较深的标本进行基因测序,测序结果与猪附红细胞体、牛附红细胞体和羊附红细胞体16S rRNA基因序列的同源性分别为95.84%、86.84%和87.33%。结论多种方法联合检测可提高附红细胞体检测的特异性。

Objective To improve the determination specificity of human Eperythrozoon through multiple methods. Methods A total of 223 patients with blood diseases were enrolled. Eperythrozoon was determined by Wright-Giemsa staining and acridine staining. The determination rates were compared between the 2 methods. The cases with both positive results were determined by polymerase chain reaction(PCR). The positive results of PCR were analyzed by sequencing and GenBank comparison. Results The positive rates were 46.19% and 28.25% by Wright-Giemsa staining and acridine staining,respectively. In 40 cases with both positive results,10 cases were positive by PCR,and 1 case with dark stripe was sequenced. The homology of 16S rRNA sequences between this case and Mycoplasma suis was 95.84%,that for Mycoplasma bovis was 86.84%,and that for Mycoplasma capricolum was 87.33%. Conclusions The multiple methods for Eperythrozoon could improve determination specificity.