多囊卵巢综合征患者血清HIF1α、VEGF水平及胰岛素抵抗

The investigation of levels of serum HIF1α and VEGF of women with polycystic ovary syndrome and its relationship to the insulin resistance of women

目的:探讨多囊卵巢综合征(PCOS)患者血清缺氧诱导因子(HIF1α)、血管内皮生长因子(VEGF)水平及其与胰岛素抵抗(IR)的关系。方法:选取2015年6月—2017年12月本院收治的PCOS患者70例为研究对象,选择同期健康女性志愿者70例为对照组,化学发光仪检测血清胰岛素抵抗相关指标,酶联免疫吸附测定法测定血清HIF-1α、VEGF水平,采用胰岛素抵抗指数(HOMA-IR)评估IR水平, Pearson相关分析法分析血清HIF1α、VEGF水平及其与IR关系。结果:PCOS组血清黄体生成素/促卵泡激素(LH/FSH)、睾酮(T)、空腹胰岛素(FINS)、HOMA-IR,以及HIF1α、VEGF水平均高于对照组(均P<0.05);PCOS组肥胖者(BMI≥25 kg/m^2)血清HIF1α、VEGF水平及HOMA-IR均高于PCOS组非肥胖者(BMI<23 kg/m^2);PCOS患者血清HIF1α、VEGF水平与BMI、WHR、LH/FSH、T、FINS、HOMA-IR均呈正相关(P<0.05)。结论:PCOS患者血清中HIF1α、VEGF表达上调,可能作为预测PCOS患者胰岛素抵抗发生的指标。

Objective:To investigate the levels of serum hypoxia inducible factor (HIF1α) and vascular endothelial growth factor (VEGF) of women with polycystic ovary syndrome (PCOS),and to explore the relationships between the levels of serum HIF1α or VEGF and insulin resistance (IR) of women.Methods:70 women with PCOS were selected in study group from June 2015 to December 2017,and 70 healthy female volunteers were selected in control group during the same period.The related indicators of IR were detected by chemiluminescence,and the levels of serum HIF 1αand VEGF were measured by enzyme linked immunosorbent assay (ELISA).IR was evaluated by insulin resistance index (HOMA IR),and Pearson correlation analysis was used to analyze the relationships between level of serum HIF1α or VEGF and IR.Results:The value of luteinizing hormone/follicle stimulating hormone (LH/FSH),the level of testosterone (T) and fasting insulin (FINS),the value of HOMA IR,and the levels of serum HIF1α and VEGF of women in study group were significant higher than those of women in control group (P <0.05).In study group,the levels of serum HIF1 and VEGF,and the value of HOMA IR of women with BMI ≥25 kg/m 2 were significant higher than those of women with BMI <23 kg/m 2.Pearson correlation analysis showed that the levels of serum HIF1α and VEGF of women in study group were positively correlated with values of BMI,waist hip rate(WHR),LH/FSH,T,FINS,and HOMA IR (P <0.05).Conclusion:The levels of serum HIF1 and VEGF of women with PCOS are up regulated,which may be used as indicators for predicting their IR.