摘要
目的探讨宁夏地区男性生育异常与染色体多态性的关系。方法收集2016年1月至2018年5月来我院生殖中心就诊的男性生育异常患者3 102例,抽取患者外周静脉血2~4ml进行淋巴细胞培养,并进一步做G显带及染色体核型分析;对患者进行常规精液检测,根据精液结果分为精液正常组(n=2 741)、无精子症组(n=259)及少弱精子症组(n=102),比较分析三组间染色体多态性检测情况。结果患者中检出染色体多态者共计588例,检出率18.95%。染色体多态核型分析结果显示,222例(37.76%)为D/G组(包括13、14、15、21、22号染色体)随体增加;67例(11.39%)为1号、9号、16号染色体次缢痕的增加;29例(4.93%)为9号染色体臂间倒位;270例(45.92%)为Y染色体多态变异。无精子症组、少弱子精症组及精液正常组患者染色体多态检出率分别为22.01%、28.43%和18.31%,少弱精子症组染色体多态检出率显著高于其他两组(P<0.05),而无精子症组与精液正常组比较,差异无统计学意义(P>0.05)。结论染色体多态与男性无精、少精等生育异常密切相关,在临床遗传咨询和诊断治疗中应引起重视。
Objective: To analyze the relationship between male fertility abnormalities and chromosomal polymorphism in Ningxia province.Methods:Two to four ml of peripheral blood samples were collected for lymphocyte culture and further G band &karyotype analysis from 3 102 male fertility abnormalities in our fertility medicine center from January 2016 to May 2018.Routine semen analysis was performed.According to semen analysis results,the patients was divided into normal group(n=2 741),azoospermia group(n=259)and oligoasthenospermia group(n=102).The polymorphism detection was performed and compared among the three groups.Results:A total of 588 patients with chromosomal polymorphism were detected,and the detection rate was 18.95%.Chromosomal polymorphic karyotype analysis showed that 222 patients with increase of D/G group trabant,accounting for 37.76%.Among them,67 patients(11.39%)had increase of secondary constriction on chromosome 1,9,16,29,and 29 patients(4.93%)with pericentric inversion on chromosome 9,while 270 patients(45.92%)with Y chromosome polymorphism.The incidence of chromosome polymorphism in the azoospermia,oligoasthenospermia and normal groups was 22.01%,28.43% and 18.31% respectively.The incidences of chromosome polymorphism in the oligoasthenospermia group was significantly higher than that in the other two groups(P <0.05).However,there was no significant difference between the azoospermia group and the normal semen group(P>0.05).Conclusions:Chromosomal polymorphisms are closely related to male fertility abnormalities such as azoospermia and oligoasthenospermia,and the clinical genetic counseling and diagnosis and treatment should be paid attention to.
作者
宋旭梅
王贵杰
随瑞芝
王莉
詹福寿
SONG Xu-mei;WANG Gui-jie;SUI Rui-zhi;WANG Li;ZHAN Fu-shou(Department of Medical Experimental Center, General Hospital of Ningxia Medical University, Yinchuan 750001)
出处
《生殖医学杂志》
CAS
2019年第5期457-461,共5页
Journal of Reproductive Medicine
基金
宁夏医科大学科学研究基金资助项目(XY2017159)
宁夏回族自治区重点研发计划(2018BEG03030)
关键词
染色体多态性
生育异常
染色体核型分析
精液
Chromosomal polymorphism
Fertility abnormality
Chromosomal karyotype analysis
Semen