NF-κB在血栓闭塞性脉管炎患者血管壁中的表达及其意义

Investigation of correlation between expressions of NF-κB and thromboangiitis obliterans

目的探讨核因子-κB(NF-κB)在血栓闭塞性脉管炎(TAO)患者血管壁中的表达及其临床意义。方法选取2016年3月至2018年9月该院因TAO行截肢手术患者16例和非血管疾病行截肢手术患者13例作为研究对象,获得受试者的血管组织,采用免疫组织化学技术检测组织中NF-κB的表达。采用激光共聚焦技术,检测NF-κB在TAO患者血管壁中的表达特点。结果 TAO患者血管壁中NF-κB的阳性表达率为87.5%(14/16),显著高于对照组的7.7%(1/13),差异有统计学意义(P<0.01)。在TAO患者中,NF-κB主要表达于血管内皮细胞及血管平滑肌细胞。结论 NF-κB的表达与TAO的发生、发展关系密切,NF-κB信号通路诱导的炎性反应可能是TAO发病的重要因素之一。

Objective To investigate the expression and clinical significance of nuclear factor-kappa B (NF-κB) in the vessel wall of patients with thromboangiitis obliterans (TAO). Methods Sixteen patients with amputation surgery and 13 patients with non-vascular disease amputation were enrolled in the study.The vascular tissue of the subject was obtained.Immunohistochemistry was used to detect the expression of NF-κB in tissues.Laser confocal technique was used to detect the expression of NF-κB in the vessel wall of TAO patients. Results The positive expression rate of NF-κB in the vascular wall of patients with TAO was 87.5%(14/16),which was significantly higher than that of the control group (7.7%(1/13)).The difference was statistically significant ( P <0.01).In TAO patients,NF-κB is mainly expressed in vascular endothelial cells and vascular smooth muscle cells. Conclusion The expression of NF-κB is closely related to the occurrence and development of TAO.The inflammatory response induced by NF-κB signaling pathway may be one of the important factors in the pathogenesis of TAO.