18种杀菌剂对黑龙江省稻瘟病菌的室内毒力测定

Toxicity Measurement of 18 Kinds of Fungicides on Magnaporthe grisea

为筛选针对稻瘟病高效的药剂,研究采用生长速率抑制法和玻片萌发法,测定了18种杀菌剂对稻瘟病菌(Magnaporthe grisea)菌丝生长和孢子萌发的影响。结果表明:45%三苯基乙酸锡、25%腈菌唑、80%乙蒜素、25%咪鲜胺、戊唑醇(有效含量430 g·L^(-1))和75%肟菌·戊唑醇对稻瘟病菌菌丝生长具有最高的抑菌作用。其EC_(50)值分别为1.594 7,2.791 2,3.039 3,3.132 5,3.224 1,3.522 0μg·mL^(-1);25%腈菌唑、25%咪鲜胺、80%乙蒜素、45%三苯基乙酸锡对病原菌分生孢子萌发具有较高的抑制作用,其EC_(50)值分别为0.042 7,0.301 6,0.421 7,0.651 3μg·mL^(-1)。45%三苯基乙酸锡、25%腈菌唑、80%乙蒜素、25%咪鲜胺4种药剂既能抑制稻瘟病菌菌丝生长又能抑制孢子萌发。

In order to screen effective fungicides against rice blast,the effects of 18 kinds of fungicides on mycelial growth and spore germination of Magnaporthe grisea were studied by growth rate inhibition method and slide germination method.The results showed that 45% triphenyltin acetate,25% myclobutanil,80% ethylicin,25% prochloraz,tebuconazole (effective content 430 g ·L ^-1 ) and 75% emamectin benzoate could effectively inhibit mycelium growth of Magnaporthe grisea .Their EC 50 values were 1.594 7,2.791 2,3.039 3, 3.132 5 , 3.224 1 and 3.522 0 μg · mL ^-1 ,respectively;25% myclobutanil,25% prochloraz,80% ethylicin,45% triphenyltin acetate had a high inhibitory effect on the spore germination of magnaporthe grisea ,with EC 50 values of 0.042 7 ,0.301 6,0.421 7 and 0.651 3 μg ·mL ^-1 .45%Triphenyltin acetate,25% myclobutanil,80% ethylicin,and 25% prochloraz,could not only inhibit the growth of mycelium but also inhibit spore germination of Magnaporthe grisea .