慢性血栓栓塞性肺动脉高压患者血管平滑肌细胞中FoxO3a和MMP2表达升高

Increased expression of FoxO3a and MMP2 in vascular smooth muscle cells of patients with chronic thromboembolic pulmonary hypertension

目的研究FoxO3a及MMP2在慢性血栓栓塞性肺动脉高压(CTEPH)中的表达和作用。方法收集CTEPH患者20例,选取5例清洁手术标本,原代培养得到患者肺动脉血管平滑肌细胞(VSMCs);利用供体肺动脉主干作为对照,原代培养得到正常肺动脉VSMCs。免疫组织化学染色及Western blot检测血管组织及VSMCs中FoxO3a和MMP2表达。结果疾病组FoxO3a的积分吸光度值(IA)为:3 269±338,显著高于对照组的420±46(P<0.01);疾病组和对照组的MMP2的IA分别为4 936±521、1 799±139(P<0.01);FoxO3a和MMP2在疾病组表达较对照组明显升高(P<0.05)。结论 CTEPH患者肺血管中FoxO3a和MMP2高表达,这两种信号分子可能参与了CTEPH血管重塑的过程。

Objective To study the role of FoxO3a and MMP2 in chronic thromboembolic pulmonary hypertension (CTEPH). Methods Twenty patients with CTEPH admitted to the China-Japan Friendship Hospital during 2018.1-2018.12 were enrolled in this study. Pulmonary vascular smooth muscle cells (VSMCs) were obtained from 5 of 20 surgical specimens. Pulmonary artery trunk of donor was used as a control. The expression of FoxO3a and MMP2 in the surgical specimens and VSMCs from the normal group and disease group were observed by immunohistochemistry and Western blot. Results The integral absorbance value ( IA ) of FoxO3a in the CTEPH group was 3 269±338, which was significantly increased as compared with normal group 420±46 ( P <0.01);the IA of MMP2 respectively was 4 936±521, 1 799±139( P <0.01);The expression of MMP2 and FoxO3a in the CTEPH group significantly increased as compared with normal group ( P <0.05). Conclusions Overexpression of FoxO3a/MMP2 may be a potential mechanism in the vascular remodeling, thus accelerating the occurrence and development of CTEPH.