盐知母醇提物对2型糖尿病模型大鼠血糖血脂的影响

Effect of Ethanol Extract from Saline Rhizoma Anemarrhenae on Blood Glucose and Blood Lipid in Rat Models with Type 2 Diabetes Mellitus

目的优选盐知母醇提物降血糖调血脂的最佳体积分数。方法腹腔注射链脲佐菌素(STZ,40 mg/kg)合并喂饲高脂饲料,以建立2型糖尿病大鼠模型,随机分为模型组和不同体积分数醇提物组,分别给予相应药物7,14 d后测定大鼠空腹血糖(FBG),并采用酶联免疫吸附(ELISA)法测定三酰甘油(TG)、高密度脂蛋白(HDL)和低密度脂蛋白(LDL)等指标水平,并计算胰岛素敏感指数(ISI)和胰岛素抵抗指数(HOMA-IR)。结果与模型组比较,不同体积分数醇提物组大鼠FBG、血清空腹胰岛素(FINS)、总胆固醇(TC)、TG、糖化血红蛋白(HbA1C)、LDL、ISI、HOMA-IR显著降低,HDL明显升高,差异均有统计学意义(P <0. 01或P <0. 05),其中75%乙醇组上述指标均较优。结论 75%盐知母醇提物降血糖、调血脂效果最好。

Objective To optimize the optimum volume fraction of ethanol extract from saline Rhizoma Anemarrhenae for lowering blood glucose and regulating blood lipid. Methods The rat models with type 2 diabetes mellitus( T2 DM) were established by intraperitoneal injection of streptozotocin( STZ,40 mg/kg) and feeding with high-fat diet. The rats were randomly divided into the model group and ethanol extract groups with different volume fractions,7 and 14 d after the corresponding drugs were given to each group,the levels of fasting blood glucose( FBG) of rats was determined,the triglyceride( TG),high-density lipoprotein( HDL) and low-density lipoprotein( LDL) of rats were determined by ELISA,the insulin sensitivity index( ISI) and insulin resistance index( HOMA-IR) of rats were calculated.Results Compared with the model group,different volume fractions of ethanol extracts could significantly reduce the levels of serum FBG,serum fasting insulin( FINS),total cholesterd( TC),TG,glycosylated hemoglobin( HbA1 C),LDL,ISI and HOMA-IR were significantly lower,while the levels of HDL were significantly higher in the ethanol extract groups with different volume fractions,the difference was statistically significant( P < 0. 01 or P < 0. 05),of which 75% ethanol group had the best indexes. Conclusion 75% ethanol extract from saline Rhizoma Anemarrhenae has the best effect in lowering blood glucose and regulating blood lipid.