转基因小鼠源性胰腺癌组织块皮下移植瘤模型的构建与评价

Establishment of transgenic mice-derived allografts in pancreatic cancer

目的构建基于转基因小鼠的胰腺癌组织块皮下移植瘤模型(transgenic mice-derived allografts, MDAs),为胰腺癌的体内及体外研究提供良好的实验载体模型。方法利用LSL-Kras^(G12D/+);Trp53^(fl/+);Pdx1-Cre(KPC)胰腺癌转基因小鼠的组织块进行皮下移植,构建MDAs。利用HE染色、Masson染色和免疫组化染色评价MDAs的组织病理特征及免疫细胞浸润情况。结果 MDAs成功模拟出胰腺癌的大体和组织病理形态,且其保留了胰腺癌高增殖和高间质纤维化的病理特征。MDAs的纤维化和增殖程度较正常胰腺明显增高(P<0.05);MDAs还可成功模拟胰腺癌MPO+中性粒细胞、CD68+巨噬细胞和CD3+T淋巴细胞的浸润状态。结论 MDAs更利于在体内模拟胰腺癌的进展,是一种可行的胰腺癌研究模型。

Objective To establish the transgenic mice-derived allografts (MDAs) so as to provide an appropriate experiment model of pancreatic cancer. Methods By using the tissue fragments derived from transgenic mice with pancreatic cancer named LSL-Kras^G12D/+;Trp53^fl/+;Pdx1-Cre (KPC) mice and subcutaneous transplant technique,we constructed the MDAs.We evaluated the histopathological characteristics of MDAs and infiltration of immune cells by H&E staining,Masson's staining and immunohistochemical method. Results MDAs could mimic the pathological morphology,proliferation and fibrosis of pancreatic cancer precisely.Besides,the infiltration of immune cells in MDAs tumor was the same as that of pancreatic cancer. Conclusion MDAs are an effective model to mimic the development of pancreatic cancer.