甲型H1N1流行性感冒患者中miRNAs的表达研究

The expression of miRNAs in patients with A H1N1 influenza

目的探讨甲型H1N1流行性感冒患者中miRNAs表达及其临床意义。方法 2016年3月至2018年6月期间于我院就诊的168例患者作为本实验的研究对象,分为甲型H1N1流感患者组(n=86)和对照组(n=82),收集两组患者血清,检测miRNAs的表达变化。结果与对照组相比,miR-196a-5p、miR-21和miR-221在甲型H1N1流感患者血清中表达升高,miR-203、miR-502表达降低,miR-101、miR-221、miR-24、miR-27a、miR-326无差异性表达。此外,与治疗前相比,miR-196a-5p、miR-203、miR-221、miR-502在甲型H1N1流感患者治疗后没有显著差异,miR-21在甲型H1N1流感患者治疗后显著降低。结论 miR-21在甲型H1N1流行性感冒患者血清中表达升高,可以作为预测、诊断、治疗甲型H1N1流行性感冒的重要指标。

Objective To investigate the expression of miRNAs in patients with A H1N1 influenza and its clinical significance.Methods A total of 168 patients who were admitted to our hospital from March 2016 to June 2018 were enrolled in the study.They were divided into the H1N1 influenza group(n=86)and the control group(n=82).The serums of both groups were colletec to detect the dysregulated expression of miRNAs.Results Compared with the control group,miR-196a-5p,miR-21 and miR-221 were elevated in the serum of patients with A H1N1 influenza.And the expression of miR-203 and miR-502 was decreased.miR-101 and miR-221,miR-24,miR-27a,miR-326 showed no differential expression.In addition,the expression of miR-196a-5p,miR-203,miR-221,and miR-502 was not significantly different after treatment in patients with A H1N1 influenza.And miR-21 was significantly reduced after treatment in patients with A H1N1 influenza.Conclusion The expression of miR-21 in serum of patients with A H1N1 influenza is significantly elevated.And it can be used as an important indicator for predicting,diagnosing and treating A H1N1 influenza.